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  Calcium measurement methods

  edited by Alexei Verkhratsky and Ole H. Petersen.

  Contents:
  Principles of the CA2+ homeostatic/signalling system / A. Verkhratsky and O.H. Petersen
  CA2+ recordings: Hardware and software (from microscopes to cameras) / E.C. Toescu and J. Graham
  CA2+ imaging: Principles of analysis and enhancement / F. Mammano and M. Bortolozzi
  Bioluminescent CA2+ indicators / L. Fedrizzi and M. Brini
  Monitoring calcium levels with genetically encoded indicators / O. Garaschuk and O. Griesbeck
  Intracellular calcium-sensitive microelectrodes / R.C. Thomas
  CA2+ caging and uncaging / S.H. Kim and M.K. Park
  CA2+ imaging of intracellular organelles: Endoplasmic reticulum / R. Blum, O.H. Petersen and A. Verkhratsky
  CA2+ imaging of intracellular organelles: Mitochondria / L. Núñez [and others]
  CA2+ imaging of dendrites and spines / K. Holthoff
  In vivo CA2+ imaging of the living brain using multi-cell bolus loading technique / G. Eichhoff [and others]
  CA2+ imaging of glia / C. Lohr and J.W. Deitmer.

  Digital Access Springer 2010
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• Article

  Role of thyroid hormones in the normal and glucocorticosteroid hormone-induced evolution of carbamoyl-phosphate synthase (ammonia) activity in axolotl liver.

  Lamers WH, Vink C, Charles R.

  Comp Biochem Physiol B. 1978;59(2):103-10.

  1. In axolotl liver, the activity of carbamoyl-phosphate synthase (ammonia), expressed per mg liver protein, decreases to a minimum at 5 months of age, then increases to a maximum at 8 months of age which is followed by a decrease again. The initial decrease between 3 and 5 months of age appears to be largely due to an increase in non-carbamoyl-phosphate synthase protein and the following increase between 5 and 8 months of age to a relative increase of carbamoyl-phosphate synthase protein. 2. Treatment of the animals with triiodothyronine causes an increase in carbamoyl-phosphate synthase activity, the extent of which is dependent upon hormone concentration and age of the animal. After 8 months of age no increase of enzyme occurs upon thyroid hormone treatment, although metamorphosis occurs. 3. Glucocorticosteroid hormones stimulate carbamoyl-phosphate synthase activity 2-to 3-fold in animals older than 6 months. However, in animals younger than 6 months, low concentrations of thyroid hormone, insufficient to induce metamorphosis, are necessary as permissive agents. 4. The stimulatory effects of high concentrations of thyroid hormones (T3) on carbamoyl-phosphate synthase appear to be mediated via a stimulatory effect on glucocorticosteroid biosynthesis. 5. The natural rise in enzyme activity between 5 and 8 months of age seems to be due to a rise in the concentration of circulating glucocorticosteroid hormones.

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  PubMed