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  • Book
    edited by Anne Straube.
    Digital : Springer2011
    How to measure microtubule dynamics? / Anne Straube -- Purification of tubulin from porcine brain / Christopher Gell [and others] -- Purification of tubulin from the fission yeast Schizosaccharomyces pombe / Douglas R. Drummond [and others] -- Generation of differentially polyglutamylated microtubules / Benjamin Lacroix and Carsten Janke -- Preparation of pure tyrosinated or detyrosinated tubulin isoforms / Laurence Lafanechère and Didier Job -- [alpha][beta]-tubulin and microtubule-binding assays / Jaime N. Campbell and Kevin C. Slep -- Analysis of microtubule assembly kinetics using turbidimetry / Felicia Gaskin -- Isolation of centrosomes from cultured cells / Simone Reber -- Preparation of dual-color polarity-marked fluorescent microtubule seeds / Miho Katsuki, Etsuko Muto, and Robert A. Cross -- Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy / Ivo A. Telley, Peter Bieling, and Thomas Surrey -- Force generation by dynamic microtubules in vitro / Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom -- Variations on the single-molecule assay for microtubule-associated proteins and kinesins / Susanne Bechstedt [and others] -- Analysing the ATP turnover cycle of microtubule motors / Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard -- Cryo-electron tomography of microtubules assembled in vitro from purified components / Frédéric M. Coquelle [and others] -- Kinetochore microtubules, analyzed by correlated light and immunoelectron microscopy / Andreas Merdes and Jan De Mey -- Photoactivatable-GFP-[alpha]-tubulin as a tool to study microtubule plus-end turnover in living human cells / Catarina P. Samora and Andrew D. McAinsh -- Quantification of asymmetric microtubule nucleation at subcellular structures / Xiaodong Zhu and Irina Kaverina -- Investigating microtubule dynamic instability using microtubule-targeting agents / Stéphane Honore and Diane Braguer -- Laser ablation of the microtubule cytoskeleton : setting up and working with an ablation system / Nicola Maghelli and Iva M. Tolić-Nørrelykke -- Isolation, identification, and validation of microtubule-associated proteins from Drosophila embryos / Robin Antrobus and James G. Wakefield -- Isolation of novel +TIPs and their binding partners using affinity purification techniques / Ka Lou Yu [and others].