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  • Journal
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    Retired Reference (Downstairs)
    1996, 1998.
    RA975.5.P5 L55
    2
  • Article
    Rubinson KA, Baker PF.
    Proc R Soc Lond B Biol Sci. 1979 Aug 31;205(1160):323-45.
    The flow properties of axoplasm have been studied in a defined chemical environment. Axoplasm extruded from squid giant axons was introduced into porous cellulose acetate tubes of diameter roughly equal to that of the original axon. Passage of axoplasm along the tube rapidly coated the tube walls with a layer of protein. By measuring the rate of low back and forth along the tube, the rheological properties of the axoplasm plug were investigated at a range of pressures and in a variety of media. Axoplasm behaves as a classical Bingham body the motion of which can be characterized by a yield stress (theta) and a plastic viscosity (eta p). In a potassium methanesulphonate medium containing 65 nM free Ca2+, theta averaged 109 +/- 46 dyn/cm2 and eta p1 146 +/- 83 P. These values were little affected by ATP, COLCHICINE, CYTOCHOLASIN B or by replacing K by Na but were sensitive to the anion composition of the medium. The effectiveness of different anions at reducing theta and eta p1 was in the order SCN greater than I greater then Br greater than Cl greater than methanesulphonate. Theta and eta p1 were also drastically reduced by increasing the ionized Ca. This effect required millimolar amounts of Ca, was unaffected by the presence of ATP and was irreversible. It could be blocked by the protease inhibitor TLCK. E.p.r. measurements showed that within the matrix of the axoplasm gel there is a watery space that is largely unaffected by anions or calcium.
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