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- JournalSummary: Provides description of the Indian Health Service program and data on health status, demography, mortality, natality, and patient care of American Indian and Alaska native people.Digital AccessProviderVersionGoogle BooksGoogle BooksGoogle BooksGoogle BooksGoogle BooksPrint AccessLocationVersionCall NumberItems
- ArticleDornhorst A, Gann DS.J Histochem Cytochem. 1978 Nov;26(11):909-13.The unlabeled peroxidase-anti-peroxidase (PAP) method of Sternberger was used to localize cortisol within paraffin embedded sections of cat adrenal and pituitary tissue. Incubation of the cortisol antiserum used in this method with increasing concentrations of cortisol led to progressive extinction of cortisol staining of the adrenal fasciculata cells, (as measured with a scanning integrating microdensitometer). This result suggests strongly that the staining achieved with this method was specific for cortisol. Cortisol staining was demonstrated not only within cells that synthesize cortisol (the adrenal fasciculata) but also in cells of the adrenal medulla and of the anterior pituitary, two target sites for cortisol action.