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- ArticleSingh R, Singh RB.Acta Microbiol Pol. 1977;26(2):207-11.The effect of several concentrations of nitrogen (0, 21, 70, 210, 420 and 630 ppm) on the growth and multiplication of cucumber mosaic virus in cucumber (Cucumis sativus L. Barshati) grown in sand culture was studied. The effect of nitrogen nutrition on the concentration of virus appeared to be directly related to vegetative growth of the host. 420 ppm level yielding the best growth also showed the highest virus concentration and total nitrogen percentage at 30th day of infection.