Search
Filter Results
- Resource Type
- Article1
- Journal1
- Journal Digital1
- Result From
- Lane Catalog1
- PubMed1
-
Year
- Journal Title
- Acta Microbiol Pol1
Search Results
Sort by
- JournalPrintLocationVersionCall NumberItems
- ArticleBialkowska-Hobrzańska H, Kunicki-Golfinger WJ.Acta Microbiol Pol. 1977;26(1):3-8.The secondary structure of recipient DNA mated with Hfr strain was investigated by CsCl density gradient fractionation. After 45 min of HfrH64 X 3h-f-ab1157 mating one-fourth of the radioactive recipient DNA was recovered as a single-strand but only after shearing of cell lysates prior to centrifugation. This heavier than native DNA fraction of radioactive material (obtained after the first centrifugation) was degraded by single-strand specific nuclease S from Aspergillus oryzae. These findings thus confirm the authors' earlier results suggesting that in the course of mating are generated local single-stranded regions in recipient DNA.