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- ArticleRocchi A, Prantera G, Pimpinelli S, Di Castro M.Chromosoma. 1976 Jun 30;56(1):41-6.Cells of the Chinese hamster strain C-125 were treated for different time intervals with H 33258, a bibenzimidazole derivative. The same compound was used to stain fixed cells of the same strain. H 33258 induced in cells in culture specific areas of reduced spiralization on the metaphase chromosomes of some cells. These probably correspond to DNA segments rich in A-T bases interspersed along the chromosomes. Probably H 33258 acts during S period of cell cycle. The banding obtained by staining with H 33258 is similar to that induced by quinacrine dihydrochloride but shows a better resolution.