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  • Book
    Fernando C. Schmitt, editor.
    Summary: This book is intended for practicing pathologists and cytopathologists, as well as for pathology trainees and cytotechnicians. It starts with a detailed description of the extremely important pre-analytical phase for molecular testing followed by a presentation of the key tests and their application in different organs, e.g. the lung or thyroid. Step-by-step instructions for the different assays, reporting and clinical integration of the test results are discussed. The authors help the reader to benefit from their experiences by providing a valuable tool for the implementation of these techniques in daily practice. Though the use of molecular techniques is well established in surgical biopsies, to date they are not widely used in connection with cytological material. However, in some fields like lung cancer or aspirates from the pancreas and biliary tract the only available material for diagnosis is the cytological preparation a fact that has created a need for the standardization of molecular techniques on cytology. .

    Contents:
    Why Cytology for Molecular Testing? Pros and Cons
    How to Prepare Cytological Samples for Molecular Testing
    Molecular Tests Use in Cytological Material (analytical phase)
    Molecular Cytology Applications on Head and Neck
    Molecular Cytology Applications on Lung
    Molecular Cytology of Serous Effusions
    Molecular Cytology Applications on Urine
    Molecular Cytology Applications on Gynecological Cytology
    Molecular Applications in Hematolymphoid Cytology
    Molecular Cytology Application on Thyroid
    Molecular Cytology Applications on Pancreas and Biliary Tract
    Molecular Cytology Applications in Soft Tissue (Pediatric tumors)
    Molecular Cytology Applications in Metastases
    Clinical Integration of Molecular Results on Cytology (post-analytical phase).
    Digital Access Springer 2018
  • Article
    Oka A.
    J Bacteriol. 1978 Feb;133(2):916-24.
    A small plasmid (pAO2, 1 megadalton) carrying genes responsible for replication and colicin E1 immunity has been constructed from colicin E1 plasmid (A. Oka, K. Sugimoto, and M. Takanami, Proc. Mol. Biol. Jpn., p. 113-115, 1976). pAO2 DNA was cleaved into unique fragments with seven restriction endonucleases (R.HaeII,R.HaeIII,R.HapII,R.HhaI,R.AluI,R.HgaI, and R.HinfI). R.HaeII cleaved pAO2 DNA at two sites, R.HaeIII at four sites, R.HapII at nine sites, R.HhaI at eight sites, R-AluI at nine sites, R.HgaI at two sites, and R.HinfI at four sites, respectively. The order of HaeIII fragments of pAO2 was deduced from the physical map of colicin E1 plasmid previously reported (A. Oka and M. Takanami, Nature (London) 264:193-196, 1976). HapII, HhaI, and AluI fragments of pAO2 were assigned by analyzing overlapping sets of fragments arising upon digestion of individual HaeIII fragments with one of R.HapII, R.HhaI, or R.AluI, and upon their reciprocal digestion. The cleavage sites for R.HaeII, R.HgaI, and R.HinfI were localized on HapII, HhaI, and AluI fragments by combined digestion. On the basis of these data and estimates of the size of each fragment, a fine cleavage map of pAO2 was constructed.
    Digital Access Access Options