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  • Book
    Jack C. de la Torre.
    Contents:
    Chapter 1. How does Alzheimer's begin and who gets it?
    Chapter 2. Forming memories
    Chapter 3. Masquerading as dementia
    Chapter 4. Alzheimer's
    then and now
    Chapter 5. Unproven hypotheses on the cause of Alzheimer's
    Chapter 6. Other hypotheses on the cause of Alzheimer's disease
    Chapter 7. Alzheimer noise
    Chapter 8. Social contract and Alzheimer's
    Chapter 9. Genetics of Alzheimer's
    Chapter 10. Powering the brain
    Chapter 11. Pharmaceuticals and Alzheimer's
    Chapter 12. Alzheimer vascular risk factors
    Chapter 13. The Good, The Bad and The Ugly of Advanced Aging
    Chapter 14. A Personal Account of How a Scientific Hypothesis Blooms Into a Life of Its Own
    Chapter 15. Clinical tools to detect and predict individuals at risk of Alzheimer's
    Chapter 16. The turning point for Alzheimer's
    Chapter 17. How poor brain blood flow promotes Alzheimer's disease
    Chapter 18. Interventions that may increase cerebral blood flow
    Chapter 19. Great Expectations
    Chapter 20. A road to new thinking.
    Digital Access Springer 2016
  • Article
    Mehlman IJ, Eide EL, Sanders AC, Fishbein M, Aulisio CC.
    J Assoc Off Anal Chem. 1977 May;60(3):546-62.
    Surveillance for dysentery-related invasive potential in bacteria using the Sereny keratoconjunctivitis test is restricted by expense, time factor, and necessity for confirmation. Primary screening of isolates in a standardized mammalian cell culture system is recommended. Bacteria are grown 20 hr in veal infusion, washed, and resuspended in 20% heat-inactivated fetal bovine serum (FBS) supplemented with 0.12% brain heart infusion and 0.1% bile salts. The HeLa culture is grown 20 hr as a monolayer in chamber slides with 90% minimal essential medium (MEM)-10% FBS. The host culture is infected at a ratio of 10 bacteria/mammalian cell for 3 hr at 35 degrees C. The infection medium is replaced with MEM-FBS supplemented with 300 microng lysozyme and 5 microng gentamycin/ml. The infected monolayer is incubated 5 hr at 35 degrees C to permit intracellular multiplication. Specimens are washed, fixed with methanol, and stained successively with May-Grunwald and Giemsa dyes. Bacteria occur within the cytoplasm if invasion has occurred. The criterion for a positive test is that 1% of the host cells possesses at least 5 bacteria in 2 of 3 trials. Invasiveness is correlated with and possibly preconditioned by cytotoxic principle(s). Infectivity rates vary from 0 to 30%. The cytopathic effect is noted in 5-50% of HeLa cells. Positive results must be confirmed by the Sereny test.
    Digital Access Access Options