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- Bookedited by Uma Lakshmipathy, Mohan C. Vemuri.Contents:
1. Sources and derivation of human embryonic stem cells / Michal Amit
2. New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani
3. Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao
4. Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore
5. Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu, Andrew Fontes, Mohan C. Vemuri and Chad C. MacArthur
6. Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8 [trade mark] medium conditions / Andrew Fontes, Chad C. MacArthur, Pauline T. Lieu and Mohan C. Vemuri
7. Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers
8. Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins
9. Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur, Mary Lynn Tilkins, Richard Eckert and Shayne Boucher
10. Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku
11. Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
12. Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis
13. Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin
14. Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr.
15. TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel, Yalei Wu, Yun Bao, Ricardo Mancheo, Janice Au-Young and Elena Grigorenko
16. Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
17. Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete
18. Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu
19. Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner
20. Cloning technologies / Andrew Fontes
21. Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy
22. Site-specific integration in human ESC using Jump-In [trade mark] TI [trade mark] technology / Chad C. MacArthur.Digital Access Springer 2013 - ArticleKlain GJ, Hannon JP.Comp Biochem Physiol B. 1977;58(3):227-30.1. In vivo fatty acid synthesis by brown adipose tissue was enhanced in rats exposed to cold (5 degrees C) or altitude (4300 m) for 7 days but was unaltered in rats exposed to heat (35 degrees C) for an equivalent period. In vivo fatty acid synthesis by white adipose tissue was depressed by cold exposure while altitude and heat exposure had no effect. 2. In vitro, CO2 production and lipid synthesis were elevated in brown adipose tissue from rats fasted for 4 days. Refeeding (4 days) such rats reversed these effects, leading to depressed values relative to those of control rats. In contrast, these metabolic events in white adipose tissue were decreased by fasting and increased compared to controls during subsequent refeeding.