Bookedited by Uma Lakshmipathy, Mohan C. Vemuri.
Contents:
1. Sources and derivation of human embryonic stem cells / Michal Amit
2. New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani
3. Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao
4. Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore
5. Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu, Andrew Fontes, Mohan C. Vemuri and Chad C. MacArthur
6. Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8 [trade mark] medium conditions / Andrew Fontes, Chad C. MacArthur, Pauline T. Lieu and Mohan C. Vemuri
7. Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers
8. Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins
9. Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur, Mary Lynn Tilkins, Richard Eckert and Shayne Boucher
10. Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku
11. Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
12. Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis
13. Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin
14. Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr.
15. TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel, Yalei Wu, Yun Bao, Ricardo Mancheo, Janice Au-Young and Elena Grigorenko
16. Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
17. Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete
18. Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu
19. Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner
20. Cloning technologies / Andrew Fontes
21. Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy
22. Site-specific integration in human ESC using Jump-In [trade mark] TI [trade mark] technology / Chad C. MacArthur.