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  Malaria-- methods and protocols. Second edition [2nd ed.]

  edited by Robert Ménard.

  Contents:
  In vitro culturing Plasmodium falciparum erythrocytic stages / Alexander G. Maier and Melanie Rug
  Production of Plasmodium falciparum gametocytes in vitro / Kathryn Shaw Saliba and Marcelo Jacobs-Lorena
  In vitro differentiation of Plasmodium falciparum gametocytes into ookinetes / Anil K. Ghosh and Marcelo Jacobs-Lorena
  Ex vivo culture of Plasmodium vivax and Plasmodium cynomolgi and in vitro culture of Plasmodium knowlesi blood stages / Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H.M. Kocken
  Laboratory maintenance of rodent malaria parasites / Chandra Ramakrishnan [and 6 others]
  Transfection of Plasmodium falciparum / Melanie Rug and Alexander G. Maier
  Transfection of rodent malaria parasites / Nisha Philip, Rachael Orr, and Andrew P. Waters
  Recombination-mediated genetic engineering of Plasmodium berghei DNA / Claudia Pfander [and 4 others]
  Standardization in generating and reporting genetically modified rodent malaria parasites : the RMgmDB database / Shahid M. Khan [and 3 others]
  Transient transfection of Plasmodium vivax blood-stage parasites / Cecilia P. Sanchez [and 3 others]
  Silencing of genes and alleles by RNAi in Anopheles gambiae / Marina Lamacchia [and 5 others]
  Analysis of variant gene family expression by quantitative PCR / Sohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira
  DNA microarray-based genome-wide analyses of Plasmodium parasites / Zbynek Bozdech, Sachel Mok, and Archna P. Gupta
  Whole-genome analysis of Plasmodium spp. utilizing a new Agilent Technologies DNA microarray platform / Heather J. Painter [and 3 others]
  Transcriptome analysis using RNA-seq / Wieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg
  Experimental tools for the study of protein phosphorylation in Plasmodium / Dominique Dorin-Semblat [and 4 others]
  Extraction of hydrophilic metabolites from Plasmodium falciparum-infected erythrocytes for metabolomic analysis / Kellen L. Olszewski and Manuel Llinás
  Static and dynamic imaging of erythrocyte invasion and early intra-erythrocytic development in Plasmodium falciparum / David T. Riglar and Jake Baum
  Flow cytometry-based methods for measurement of cytosolic calcium and surface protein expression in Plasmodium falciparum merozoites / Shailja Singh and Chetan E. Chitnis
  Microsphiltration : a microsphere matrix to explore erythrocyte deformability / Catherine Lavazec [and 7 others]
  Atomic force microscopy of Plasmodium-infected red blood cells : detecting and localizing single molecular recognition events / Ang Li [and 3 others]
  Expressing full-length functional PfEMP1 proteins in the HEK293 expression system / Anand Srivastava, Yves Durocher, and Benoît Gamain
  Genome-wide chromatin immunoprecipitation-sequencing in Plasmodium / Jose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf
  In situ fluorescence visualization of transcription sites and genomic loci in blood stages of Plasmodium falciparum / Liliana Mancio-Silva and Artur Scherf
  Bioluminescence imaging of P. berghei schizont sequestration in rodents / Joanna Braks [and 5 others]
  Scoring sporozoite motility / Allison F. Carey, Robert Ménard, and Daniel Y. Bargieri
  Quantification of sporozoite invasion, migration, and development by microscopy and flow cytometry / Photini Sinnis [and 4 others]
  Imaging sporozoite cell traversal in the liver of mice / Joana Tavares [and 4 others]
  Analysis of liver stage development in and merozoite release from hepatocytes / Rebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler
  Quantitative analysis of Plasmodium berghei liver stages by bioluminescence imaging / Takeshi Annoura [and 4 others]
  Activation of human NK cells by Plasmodium-infected red blood cells / Amir Horowitz and Eleanor M. Riley
  Chimeric parasites as tools to study Plasmodium immunology and assess malaria vaccines / Ian Cockburn
  Development and use of TCR transgenic mice for malaria immunology research / Yun-Chi Chen and Fidel Zavala
  Tracking the total CD8 T cell response following whole Plasmodium vaccination / Katherine L. Doll, Noah S. Butler, and John T. Harty
  Screening inhibitors of P. berghei blood stages using bioluminescent reporter parasites / Jing-wen Lin [and 5 others]
  Screening and evaluation of inhibitors of Plasmodium falciparum merozoite egress and invasion using cytometry / Anthony Bouillon [and 3 others]
  Induction of anti-Plasmodium immunity following subpatent infection with live erythrocytic stages and drug cure / Danielle I. Stanisic and Michael F. Good
  Vaccination using radiation- or genetically attenuated live sporozoites / Ashley M. Vaughan and Stefan H.I. Kappe
  Vaccination using normal live sporozoites under drug treatment / Laurent Rénia [and 3 others]
  Assessing transmission blockade in Plasmodium spp. / Andrew M. Blagborough [and 5 others]
  Mosquito transgenic technologies to reduce Plasmodium transmission / Silke Fuchs, Tony Nolan, and Andrea Crisanti.

  Digital Access Springer 2012
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• Article

  Reduction of distress vocalization in chicks by opiate-like peptides.

  Panksepp J, Vilberg T, Bean NJ, Coy DH, Kastin AJ.

  Brain Res Bull. 1978 Nov-Dec;3(6):663-7.

  All the opiate-like peptides we tested (Met-enkephalin, (D-Ala2)-Met-enkephalin-NH2, beta-endorphin, (D-Ala2)-beta-endorphin, (D-Ala2)-alpha-endorphin, (D-Ala2)-gamma-endorphin) were capable of reducing distress vocalizations (DV's) in socially-isolated chicks when injected into the vicinity of the fourth ventricle in doses as low as 100 picomoles. All of these substances were at least as potent as equimolar doses of morphine sulfate. In general, DV's were a more sensitive measure of opiate-like peptide effects than reductions in body temperature. In a more limited study using peripheral injections, it was determined that (D-Ala2)-Met-enkephalin at doses of 400 nanomoles/kg, like morphine sulfate, was more effective in reducing DV's, than an equimolar dose of beta-endorphin. beta-endorphin was not as effective via a peripheral route as it was via central administration.

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  PubMed