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- Bookedited by Chrissa Kioussi.Contents:
Histological analysis of the embryonic and adult tooth / Atsushi Ohazama
Determination of gene expression patterns by whole-mount in situ hybridization / Sergiy Kyryachenko [and others]
Determination of gene expression patterns by in situ hybridization in sections/ Kateryna Kyrylkova [and others]
Immunohistochemistry and detection of proliferating cells by BrdU / Sergiy Kyryachenko [and others]
Detection of apoptosis by TUNEL assay / Kateryna Kyrylkova [and others]
Use of siRNA in dental tissue-derived cell cultures : integrin knockdown in fibroblasts / Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad
Organ cultures and kidney-capsule grafting of tooth germs / Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada
Evaluation of skull and tooth morphology and mineralization using high-resolution x-ray tomography / Brian K. Bay
Electron microscopy / Hans U. Luder and Margrit Amstad-Jossi
Deoxyoligonucleotide microarrays for gene expression profiling in murine tooth germs / Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin
Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament / Kentaro Akiyama [and others]
In vivo transplantation and tooth repair / Shuhei Tsuchiya and Masaki J. Honda
Methods to validate tooth-supporting regenerative therapies / Miguel Padial-Molina [and others]
Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method) / Masamitsu Oshima [and others]
In vitro studies on odontogenic tumors / Javier Caton, Thimios A. Mitsiadis, and Peter R. Morgan
Whole mount immunohistochemistry and in situ hybridization of larval and adultzZebra fish dental tissues / Barbara Verstraeten, Ellen Sanders, and Ann Huysseune.Digital Access Springer 2012 - ArticleBraunger R, Kling H, Krone W, Schmid M, Olert J.Hum Genet. 1977 Aug 31;38(1):65-75.A strain of fibroblasts partially trisomic for the larger part of 1q (Norwood and Hoehn, 1974) contains about 1.5 times as much fumarate hydratase (FH) as various control-strains. This gene dosage effect was ascertained by (1) comparative measurements of the specific activity; (2) relating the specific activity of FH to that of reference enzymes, not influenced by the chromosomal anomaly; and (3) by immunoprecipitation methods, using a rabbit antiserum against pig heart FH which cross-reacts with the human enzyme. Among others, this gene dosage effect can be demonstrated numerically by the following parameters: Ratio of the average specific activity of FH in the trisomic strain to that of the control strains: 1.53. Corresponding ratio after dividing FH activity by that of reference enzymes; for acid phosphatase: 1.58, for glutamate dehydrogenase: 1.53. Average ratio of the immunoprecipitation areas obtained upon radial immunodiffusion according to Mancini et al. (1965): 1.56.