Bookedited by Volkmar Weissig, Marvin Edeas.
Summary: This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. "Mitochondrial Medicine: Volume I, Probing Mitochondrial Function" focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, "Mitochondrial Medicine" provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.
Contents:
Preparation of "functional' mitochondria : a challenging business / Stefan Lehr, Sonja Hartwig, and Jorg Kotzka
Isolation and quality control of functional mitochondria / Sonja Hartwig, Jorg Kotzka, and Stefan Lehr
Isolation of mitochondria-associated ER membranes (MAMs) and glycosphingolipid-enriched microdomains (GEMs) from brain tissues and neuronal cells / Ida Annunziata, Annette Patterson, and Alessandra d'Azzo
Fluorescence analysis of single mitochondria with nanofluidic channels / Ted Pham [and 3 others]
Optical microwell arrays for large-scale studies of single mitochondria metabolic responses / Venkata Suresh Vajrala [and 4 others]
Deep resequencing of mitochondrial DNA / Brendan A.I.Payne [and 3 others]
Single-cell analysis of mitochondrial DNA / Brendan A.I.Payne, Lynsey Cree, and Patrick F. Chinnery
A high-throughput next-generation sequencing assay for the mitochondrial genome / Shale Dames, Karen Eilbeck, and Rong Mao
Rapid mitochondrial DNA isolation method for direct sequencing / Wilber Quispe-Tintaya [and 4 others]
Analysis of mitochondrial DNA and RNA integrity by a real-time qPCR-based method / Wei Wang [and 3 others]
Mitochondria-targeted RNA import / Geng Wang [and 4 others]
Accurate measurement of circulating mitochondrial DNA content from human blood samples using real-time quantitative PCR / Saima Ajaz, Anna Czajka, and Afshan Malik
mTRIP : an imaging tool to investigate mitochondrial DNA dynamics in physiology and disease at the single-cell resolution / Laurent Chatre and Miria Ricchetti
Simultaneous quantification of mitochondrial ATP and ROS production / Liping Yu, Brian D. Fink, and William I. Sivitz
Live-cell assessment of mitochondrial reactive oxygen species using dihydroethidine /Marleen Forkink [and 3 others]
Detection and differentiation between peroxynitrite and hydroperoxides using mitochondria-targeted arylboronic acid / Jacek Zielonka [and 3 others]
Time-resolved spectrometry of mitochondrial NAD(P)H fluorescence and its applications for evaluating the oxidative state in living cells / Julia Horilova [and 5 others]
Novel methods for measuring the mitochondrial membrane potential / Roger Springett
High-throughput real-time analysis of cell oxygenation using intracellular oxygen-sensitive probes / James Hynes and Conn Carey
In vivo aassessment of mitochondrial oxygen consumption / Floor A. Harms and Egbert G. Mik
Imaging mitochondrial hydrogen peroxide in living cells / Alexander R. Lippert, Bryan C. Dickinson, and Elizabeth J. New
Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production / Gerhard Kumschnabel [and 6 others]
Measurement of mitochondrial NADH and FAD autofluorescence in live cells / Fernando Bartolomé and Andrey Y. Abramov
Mitochondrial coenzyme Q10 determination via isotope dilution liquid chromatography tandem mass spectrometry / Outi Itkonen and Ursula Turpeinen
Assessing the bioenergetic profile of human pluripotent stem cells / Vanessa Pfiffer and Alessandro Prigione
Integrative methods for studying cardiac energetics / Philippe Kiole [and 7 others]
Computer-based prediction of mitochondria-targeting peptides / Pier Luigi Martelli [and 4 others]
Prediction of mitochondrial protein function by comparative physiology and phylogenetic profiling / Yiming Cheng and Fabiana Perocchi
Assessment of posttranslational modification of mitochondrial proteins / Sudharsana R. Ande, G. Pauline Padilla-Meier, and Suresh Mishra
Assessment of mitochondrial protein glutathionylation as a signaling for CO pathway / Ana S. Almeida and Helena L.A. Vieira.
High-resolution melting analysis for identifying sequence variations in nuclear genes for assembly factors and structural subunits of cytochrome C oxidase / Alžběta Vondráčková [and 3 others]
Heterologous inferential analysis (HIA) as a method to understand the role of mitochondrial rRNA mutations in pathogenesis / Joanna L. Elson, Paul M. Smith, and Antón Vila-Sanjurjo
Analysis of mitochondrial dysfunction during cell death / Vladimir Gogvadze, Sten Orrenius, and Boris Zhivotovsky
The use of FLIM-FRET for the detection of mitochondria-associated protein interactions / Elizabeth J. Osterlund, Qian Liu, and David W. Andrews
Assessment of mitochondrial Ca²⁺ uptake / András T. Deak [and 6 others]
Qualitative characterization of the rat liver mitochondrial lipidome using all ion fragmentation on an exactive benchtop orbitrap MS / Susan S. Bird [and 4 others]
Characterization of mitochondrial populations during stem cell differentiation / Petra Kerscher [and 4 others]
An ex vivo model for studying mitochondrial trafficking in neurons / Helena Bros, Raluca Niesner, and Carmen Infante-Duarte.