Bookedited by Nathalie Beaujean, Hélène Jammes, Alice Jouneau.
Contents:
Nuclear Transfer in the Mouse
Nuclear Transfer in the Rabbit
Nuclear Transfer in Ruminants
Nuclear Transfer and Transgenesis in the Pig
Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation
Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte
Assessing the Quality of Donor Cells: Karyotyping Methods
Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer
Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos
Visualization of Epigenetic Modifications in Preimplantation Embryos
Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer
Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming
Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining
Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR)
Studying Bovine Early Embryo Transcriptome by Microarray
Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level
Micro Chromatin Immunoprecipitation ({uF06D}ChIP) from Early Mammalian Embryos
Assessing Reprogramming by Chimera Formation and Tetraploid Complementation
Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology
Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP).