Search
Filter Results
- Resource Type
- Article1
- Book1
- Book Print1
- Print1
- Result From
- Lane Catalog1
- PubMed1
-
Year
- Journal Title
- Steroids1
Search Results
Sort by
- Booka report of the Surgeon General, 1985.Access via DHHS publication ; 1985; 85-50207(PHS) Health consequences of smoking ; 1985LocationVersionCall NumberItems
- ArticleBlomquist CH, Kotts CE.Steroids. 1978 Oct;32(3):399-419.When microsomes were prepared in 2-mercaptoethanol Vmax for 17beta-hydroxysteroid oxidoreductase (17beta-HSD) was greater, the Km for NAD+ was greater and the Km for testosterone lower than in its absence. During storage at 4 degrees Vmax increased in the presence of 2-mercaptoethanol and decreased in its absence; Km values for testosterone and NAD+ increased during storage in both cases. The presence or absence of 2-mercaptoethanol did not affect the extent or time-course of inactivation of 17beta-HSD by trypsin or phospholipase A. Furthermore, no differences were detected in sedimentation properties on sucrose density gradients suggesting that the differences and changes in the kinetic behavior of 17beta-HSD reflect a conformational flexibility at the active site and are not due to extensive changes in the structure of the microsomes. 17beta-HSD exposed to 2-mercaptoethanol was subject to substrate inhibition by testosterone, a type of inhibition not previously reported for this enzyme.