Search

Search Results

• Journal

  Yakhteh

  Digital Access Cell J v. 1-12, 1999-2010.
  Get Shareable Link

  View Details
• Article

  Separation and partial characterization of fractions derived from frog lung homogenates. A possible marker system for amphibian pulmonary surfactant.

  Hitchcock KR, Parsons WJ.

  J Histochem Cytochem. 1977 Dec;25(12):1363-7.

  The purpose of this study is to determine if inframammalian vertebrate (amphibian) lung contains certain nonspecific esterases that have been identified as enzyme markers for mammalian (rat and mouse) pulmonary surfactant. Density gradient centrifugation procedures were utilized to concentrate any surface-active material in frog lung homogenates. Lipid and protein analyses of one of the derived fractions and of pulmonary lavage fluid were consistent with other techniques indicating that these preparations were surface active. A comparison of the nonspecific esterases in the derived fractions and the pulmonary lavage fluid allowed the identification of a nonspecific esterase that has an electrophoretic mobility comparable to one of the nonspecific esterases already identified as an enzyme marker for mammalian (rat and mouse) pulmonary surfactant. These results indicate that these enzyme markers may be useful in the further investigation of the surfactant systems of other inframammalian vertebrates.

  Digital Access Access Options
  Get Shareable Link

  PubMed