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  • Book
    edited by Nader Francis, Robin H. Kennedy, Olle Ljungqvist, Monty G. Mythen.
    Summary: This textbook explores the latest trends in enhanced recovery from colorectal surgery, covering post-surgical fluid management and more, with examples of enhanced recovery pathways and tips on pain control, feeding, mobilisation and criteria for discharge.

    Contents:
    1.Overview: Key Elements and the Impact of Enhanced Recovery Care / Kenneth C.H. Fearon
    2.Preoperative Optimisation and Conditioning of Expectations / Jennie L. Burch
    3.The Metabolic Stress Response and Enhanced Recovery / Olle Ljungqvist
    4.Anaesthetic Contributions in Enhanced Recovery / Michael Scott
    5.Perioperative Fluid Management in Enhanced Recovery / Dileep N. Lobo
    6.Pain Control After Surgery / William J. Fawcett
    7.Colorectal Surgery and Enhanced Recovery / Alan F. Horgan
    8.Setting Up an Enhanced Recovery Programme / Robin H. Kennedy
    9.The Role of the Enhanced Recovery Facilitator / Fiona Carter
    10.Success and Failure in Colorectal Enhanced Recovery / Jonathan Ockrim
    11.Data Collection and Audit / Olle Ljungqvist.
    Digital Access Springer 2012
  • Article
    Kondorosi E, Nagy J, Dénes G.
    J Immunol Methods. 1977;16(1):1-13.
    The separation of rat T lymphocytes was investigated on anti-Ig--Ig columns. A simple and efficient method for the purification of rat Ig by precipitation of rat serum with sodium sulfate is presented. Protein binding characteristics of glass and plastic beads, as solid support of affinity columns, are described, as well as optimal parameters for coating beads with rat Ig (with BSA, ribonuclease and lysozyme, as comparison). Binding of Ig was primarily dependent on the concentration of the Ig solution. Maximal strong binding of Ig (6.2 X 10(3) molecules per micron2 of bead surface) was reached a 400 microng per ml concentration of purified Ig solution during 20 min of incubation. Higher concentrations increased only the amount of loosely bound Ig on the surface of beads whereas the amount of firmly bound Ig remained unchanged. Fractionation of lymphoid cell suspensions on anti-Ig--Ig affinity columns prepared at optimal conditions resulted in highly purified T-cell suspensions containing less than 1% of lymphocytes bearing surface Ig receptors.
    Digital Access Access Options