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- JournalDigital AccessProviderVersionWileyPEP Archivev. 1-, 1955- Full text delayed 3+ yearsAcad Search Premv. 1-, 1955- Full text delayed 12 months
- ArticleGoldstein L, Boylan JM, Boyd TA.Curr Probl Clin Biochem. 1977 Oct 23-26;8:273-9.alpha-Ketoglutarate (KG) is a potent inhibitor of renal mitochondrial glutamine transport and deamidation at physiological concentrations. The concentration-inhibition relationship observed between KG and mitochondrial glutamine transport and deamidation indicates that changes in renal KG concentration occurring during alterations in acid-base balance would have significant effects on mitochondrial ammonia production in vivo. A rapid-mixing, rapid-filtration method was developed to measure the effects of KG on initial rates of mitochondrial glutamine transport. Transport was linear for only a few seconds and approached steady-state conditions by 30 seconds. KG (0.3 mM) raised the glutamine transport Km approximately two-fold. We propose that KG may play a major regulatory role in the early adaptation of renal ammonia production to acidosis.