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  The Alabama M.D

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  v. [3, 5-7, 9, 11-14], [1967, 1969-71, 1973, 1975-78].

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  Isolation of mammalian cell mutants deficient in glucose 6-phosphate dehydrogenase by means of a replica-plating technique.

  Wild D, Hellkuhl B.

  Hum Genet. 1976 Jun 29;32(3):315-22.

  A replica-plating technique is described which was used for the isolation of G-6-PD-deficient mutants in cultures of mutagen-treated Chinese hamster cells. Mutants were recognized by their failure to stain in a histochemical G-6-PD-specific staining reaction. Four mutants were isolated and characterized by growth properties, stability of their variant phenotypes, and reduced G-6-PD activity. One of these mutants on electrophoresis exhibited a variant G-6-PD and thus is very likely the result of a mutation in the structural gene for G-6-PD.

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  Resolución integral del problema agrario en Lombardia y Nueva Italia, Michoacán.

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