Book[edited by] S. Kim Suvarna, Christopher Layton, John D. Bancroft.
Summary: This is a brand new edition of the leading reference work on histological techniques. It is an resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. New to this edition: Brand new co-editor. Self assessment questions and answers. Will help reinforce all of the basics in order to pass course exams, professional certification exams. New material on immunohistochemical and molecular diagnostic techniques. Enables user to keep abreast of latest advances in the field.
Contents:
Managing the laboratory / Louise Dunk
Safety and ergonomics in the laboratory / John D. Bancroft
Light microscopy / John D. Bancroft and Alton D. Floyd
Fixation of tissues / Anthony Rhodes
The gross room/surgical cut-up / S. Kim Suvarna and Christopher Layton
Tissue processing and microarray / Lena T. Spencer, John D. Bancroft, and Wanda Grace Jones
Microtomy : paraffin and frozen / Lena T. Spencer and John D. Bancroft
Plastic embedding for light microscopy / Neil M. Hand
How histological stains work / Richard W. Horobin
The hematoxylins and eosin / John D. Bancroft and Christopher Layton
Connective and mesenchymal tissues with their stains / John D. Bancroft and Christopher Layton
Carbohydrates / Christopher Layton and John D. Bancroft
Pigments and minerals / Guy E. Orchard
Amyloid / Janet A. Gilbertson and Toby Hunt
Microorganisms / Jeanine H. Bartlett
Bone / Diane L. Sterchi
Techniques in neuropathology / J. Robin Highley and Nicky Sullivan
Immunohistochemical techniques / Peter Jackson and David Blythe
Immunofluorescent techniques / Graeme Wild
Immunohistochemistry quality control / Tracy Sanderson and Gregory Zardin
Molecular pathology / Diane L. Sterchi and Caroline Astbury
Transmission electron microscopy / Anthony E. Woods and John W. Stirling
Quantittive data from microscopic specimens / Alton D. Floyd
Appendix I : classical histochemical methods / John D. Bancroft and Jennifer H. Stonard
Appendix II : applications of immunohistochemistry / Laura J. Keeling and Sherin Jos Payyappilly
Appendix III : measurement units / Jennifer H. Stonard
Appendix IV : preparation of solutions / Danielle Maddocks
Appendix V : buffer solutions / Paul Samuel
Appendix VI : solubility of some common reagents and dyes / Stuart Inglut
Appendix VII : mounting media and slide coatings / Ann Michelle Moon
Appendix VIII : Molecular pathology reagents / Diane L. Sterchi.