Search
Filter Results
- Resource Type
- Article1
- Journal1
- Journal Digital1
- Result From
- Lane Catalog1
- PubMed1
-
Year
- Journal Title
- Eur J Biochem1
Search Results
Sort by
- ArticlePedone F, Ballario P, Di Mauro E.Eur J Biochem. 1978 Jul 03;87(3):445-51.The number and the distribution of the sites of Escherichia coli DNA that form stable complexes with the homologous RNA polymerase (class A sites according to Hinkle and Chamberlin [3]) have been investigated. Almost all the DNA can bind RNA polymerase, even when fragmented at short (undergenic) size; this general non-promoter-specific binding is highly labile and is not temperature-dependent. The range of RNA polymerase/DNA ratios that give rise to the stable temperature-dependent complexes was examined. The amount and the distribution of class A complexes were studied analysing the dissociation of complexes formed by RNA polymerase on DNA fragments of various length. The E. coli genome appears to form 3.8 X 10(3) stable complexes; the majority of these complexes shows a short-range distribution (800-1200 base pairs). The rest is more widely spaced (1200-6000 base pairs).