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  • Book
    Yi-Wei Tang, Charles W. Stratton, editors.
    Summary: In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.

    Contents:
    Bacterial identification based on universal gene amplification and sequencing
    Molecular techniques for blood and blood product screening
    Molecular diagnostics of sexual transmitted disease
    Advances in the diagnosis of Mycobacterium tuberculosis
    Advanced typing techniques in molecular epidemiology investigations
    Molecular detection and characterization of carbapenem-resistant Enterobacteriaceae
    Rapid Screening and Identification of MRSA
    Advanced methods for detection of food-borne pathogens
    Technical and clinical niches for point of care molecular devices
    Molecular diagnosis of emerging coronavirus infections
    Technical advances in veterinary diagnostic microbiology
    Recent advances in veterinary diagnostic virology
    Deep sequencing: technical advances and clinical microbiology applications
    Splicing RNA and application in clinical microbiology
    Application of microarrays for laboratory diagnosis of fungal infections
    Laboratory technical advances in diagnosis of Clostridium difficile infections
    Clostridium difficile infections in one health
    Technical advances in molecular diagnostics of HIV-1 infections
    Multiplex techniques for detection and identification of microbial pathogens
    Molecular diagnosis and monitoring of human papillomavirus infections
    Molecular niches for laboratory diagnosis of sepsis
    Advanced pathology techniques for emerging infectious disease pathogens
    Diagnosis and assessment of microbial infections with host miRNA profiles
    Microbiome in diagnosis and monitoring of microbial infections
    Whole genome sequencing for microbial pathogen detection and identificaiton
    Host immune repertoire and microbial infections
    Advanced techniques for antiviral drug resistance determination
    Test algorithms using advanced techniques
    Clinical interpretation and relevance of advanced technique results.
    Digital Access Springer 2018
  • Article
    Katsuyama T, Spicer SS.
    J Histochem Cytochem. 1978 Apr;26(4):233-50.
    Various treatments carried out prior to the concanavalin A-horseradish perioxidase (HRP) method have been found to affect the staining and have permitted differentiation of three main classes of complex carbohydrates in the rat alimentary tract. Class I mucosubstances lose and class II and III paradoxically gain concanavalin A-horseradish peroxidase reactivity after periodate oxidation. Class II mucosubstances lose whereas class III retain or increase their reactivity with a reduction step interposed between oxidation and concanavalin A-horseradish peroxidase staining. Mucous neck cells, pyloric glands, Brunner's glands and mast cells exhibit strong class III staining, whereas other sites such as intestinal goblet and salivary gland acini differ widely in their type of staining. Liver glycogen stains like mucosubstances in an unstable subgroup of class III. The paradoxical increase in concanavalin A binding during oxidation correlates with the appearance of Schiff reactivity implicating oxidation of vicinal hydroxyls as the basis for the effect. The periodate-induced staining is therefore, thought to result from an oxidative disruption of linkages between vicinal hydroxyls of neighboring sugars and hydroxyls of mannose required for concanavalin A binding. Staining with the described concanavalin A-horseradish peroxidase variants appears to afford information concerning cytochemical distribution of mannose-rich glycoproteins as well as differences among these substances in the relation of mannose to neighboring sugars.
    Digital Access Access Options