BookVijay Kumar, Kiran Dip Gill.
Summary: This book is a practical guidebook in biochemistry, for medical as well as life sciences' students. The book covers reference values, sample collection procedure and detailed protocol to perform experiments. Each experiment starts with a brief introduction of the protocol, followed by specimen requirements and procedure. The procedures are presented in a very lucid manner and discuss details of calculations and clinical interpretations, The book is divided into 29 chapters, It offers references, general guidelines and abbreviations and provides principles and procedures of clinical biochemistry tests, along with their diagnostic importance.
Contents:
Common clinical laboratory hazards and waste disposal
Blood collection and preservation
Quality Control in laboratory
Automation in clinical laboratory
Photometry-Colorimeter and Spectrophotometer
Preparation of general laboratory solutions and buffers
Examination of urine for normal constituents
To perform qualitative tests for urinary proteins
To determine the quantity of proteins in urine sample using Biuret reaction
To estimate the amount of total protein and albumin in serum and to find A/G ratio
To perform qualitative test for reducing substances in urine
Quantitative analysis of reducing sugars in urine
Estimation of blood glucose levels by Glucose Oxidase method
Estimation of blood glucose levels by Folin and Wu method
To perform Glucose Tolerance Test
Estimation of urea in serum and urine
To determine urea clearance
To estimate creatinine level in serum and urine by Jaffe's reaction
To determine creatinine clearance
To determine the uric acid concentration in serum and urine
Estimation of total calcium in serum and urine
Estimation of inorganic phosphorus in serum and urine
To estimate the amount of total cholesterol in serum
To estimate total and direct bilirubin in serum
To determine Alanine and Aspartate Transaminases activity in serum
To estimate the activity of alkaline phosphatase in serum
To estimate the activity of acid phosphatase in serum
To determine serum and urinary amylase activity
To estimate the activity of lipase in serum
Qualitative analysis of ketone bodies in urine
Qualitative test for bile pigments and urobilinogen in urine
Determination of total lactate dehydrogenase activity in serum
To measure the activity of Creatine kinase-MB and Total Creatine kinase in serum
Analysis of Cerebrospinal Fluid for proteins and sugars
To analyze lipid profile from given serum sample
To determine sodium and potassium in serum by using Flame photometry
To perform Radioimmunoassay
To perform Enzyme linked Immunosorbant assay
Some important case studies.