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  Studies on column fractionation of immune cells. VII. Electrophoretic mobility and functional behavior of mouse spleen cells after fractionation on a weakly basic ion exchanger.

  Eckert R, Jenssen HL, Köhler H, von Broen B, Pasternak G.

  Acta Biol Med Ger. 1978;37(9):1393-7.

  After passing normal mouse spleen cells through columns of a weakly basic ion-exchanger, the percentage of cells with B cell-typical electrophoretic mobility (EPM) is 90% after fractionation at pH 8.0 and 10--20% at pH 5.0. However, the characterization of fractionated cells with regard to theta-antigen as well as graft-versus-host reaction shows that separation of T- and B-lymphocytes had not occurred. After incubation of the separated cells for 2 h at 37 degrees C the EPM returned to that of unseparated cells. It appears that the net charge of T- and B-lymphocytes is changeable depending on pH, temperature and separation material.

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  Collegium pathologicum

  calamo exceptum a Ioanne Friderici Hufeland, Jaennstadio-Thur. Anno MDCCXLIX.

  Summary: These two works appear to be transcriptions or excerpta taken from unidentified or unpublished works by either Hamberger or Hufeland; although Hamberger appears to be author, he is much older than Hufeland. The first work includes manuscript signatures A-M supporting that it was copied from a published work.
  
  Contents:
  [pt. 1] 1. De pathologia in genere
  2. De causis mediatis et remotiosibus
  3. De causis mediatis proximioribus
  4. De morbis eorum[que] differentiis extentiabitus
  5. De differentiis morborum accidentalibus
  6. De symptomatibus.

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  Medical History Center: By appointment only.

  [1749]

  J24H .H19 1749

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