Bookedited by Zsolt Peter Nagy, Alex C. Varghese, Ashok Agarwal.
Summary: Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.
Contents:
Assisted reproductive technologies to prevent transmission of mitochondrial DNA disease
Nuclear and Cytoplasmic Transfer: Human Applications and Concerns
Embryo culture and phenotype of the offspring
Traceability in ART
The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development
Cytoskeletal architecture of human oocytes with a focus on centrosomes and their significant role in fertilization
Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence
Livestock Production via Micromanipulation endorf Micromanipulator: Setup and Operation of Electronic Micromanipulators.-Oocyte Treatment and Preparation for Microinjection
Piezo-ICSI
Traditional Embryo Morphology Evaluation: From the Zygote to the Blastocyst Stage
Predicting Embryo Implantation Potential Using Video Monitoring By the Embryoscope Time-Lapse System
Predicting Embryo Developmental Potential and Viability Using Automated Time-Lapse Analysis
Proteomics and Metabolomics
Amino Acid Turnover as a Biomarker of Embryo Viability
Microfluidics and Microanalytics to Facilitate Quantitative Assessment of Human Embryo Physiology
Assisted Hatching of Human Embryos for Successful Implantation
Polar Body Biopsy
Cleavage-Stage Embryo Biopsy
Current and Novel Methods for Chromosome Testing.-Embryo Biopsy for PGD: Current Perspective
Sperm Cryopreservation
Technologies of Cryoprotectant-free Vitrification of Human Spermatozoa: Asepticity as Criterionn of Effectiveness
Slow Freezing of Oocytes
Vitrification: Methods Contributing to Successful Cyopreservation Outcomes
Oocyte Vitrification and Current Clinical Applications
Slow Freezing of Embryos
Vitrification of Embryos
Ovarian Tissue Cryopreservation
Establishing and Managing Donor Oocyte Banking
Single Embryo Transfer
Embryo Transfer: Techniques and Trouble shooting
G-CSF and GM-CSF: Clinical applications in reproductive medicine
The Freeze All Cycle: A New Paradigm Shift in ART
IVF Data Management: From Clipboards to Smart Apps
Regulation of the ART Laboratory
Legislation in the UK
Regulation, Licensing, and Accreditation of the ART Laboratory in Europe
Regulation, Licensing, and Accreditation of the ART Laboratory in Brazil
Regulation, Licensing and Accreditation of ART Laboratories in Australia
Trouble Shooting In Ivf Laboratories
Benchmarking and Decision Making in the IVF Laboratory
Automation, Artificial Intelligence and innovations in the future of IVF
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