Bookedited by Arun K. Shukla.
Contents:
Part A: Targeted m6A reader proteins to study the epitranscriptome / Simone Rauch and Bryan C. Dickinson
Synthetic gene expression circuits regulating sexual reproduction / Nobuo Fukuda and Shinya Honda
Nucleotide resolution sequencing of N4-acetylcytidine in RNA / Justin M. Thomas, Keri M. Bryson and Jordan L. Meier
A guide for drug inducible genome editing with HIT systems / Chen Zhao, Shixian Wei and Yu Wang
A guide for drug inducible transcriptional activation with HIT systems / Chen Zhao, Shixian Wei and Yu Wang
Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination / Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore
Salt-sensitive intein for large-scale polypeptide production / Yi-Zong Lee and Shih-Che Sue
Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls / M. Escarlet Díaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grünberg
Design, cloning and characterization of transcription factor-based inducible gene expression systems / Erik K.R. Hanko, Nigel P. Minton and Naglis Malys
Design, construction, and validation of optogenetic proteins / Colin P. O'Banion, Anwesha Goswami and David S. Lawrence
Overcoming component limitations in synthetic biology through transposon-mediated protein engineering / Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg
Chemical biology of glycoproteins: From chemical synthesis to biological impact / Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan
In bulla functional channel expression systems that mimic bacterial synthetic membranes / Masayuki Iwamoto and Shigetoshi Oiki
A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems / John E. Gadbery and Nicole S. Sampson
Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases / David Albesa-Jové, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin
Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy / Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia
Improved sensitivity and resolution of in-cell NMR spectra / David S. Burz, Leonard Breindel and Alexander Shekhtman
High-throughput methods in aptamer discovery and analysis / Kyle H. Cole and Andrej Lupták
Drop-in-well chamber for droplet interface bilayer with built-in electrodes / Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki. Part B: Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systems / Brianna E.K. Jayanthi, Wenting Zhao and Laura Segatori
Design and synthesis of fluorescent activity probes for protein phosphatases / Garrett R. Casey, Jon R. Beck and Cliff I. Stains
Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells / Travis J. Nelson, Jia Zhao and Cliff I. Stains
Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation / Justin Lawrie, Wei Niu and Jiantao Guo
Synthesis and screening of bead-displayed combinatorial libraries / Todd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek
Visualizing the brain's astrocytes / Alyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin
Modular activatable bioorthogonal reagents / Pratik Kumar and Scott T. Laughlin
Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptors / Sam Lear, Zaid Amso and Weijun Shen
Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes / Lyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi
Selection and optimization of enzyme reporters for chemical cytometry / Angela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton
Synthetic cell-like membrane interfaces for probing dynamic protein-lipid interactions / Spencer T. Glantz, Erin E. Berlew and Brian Y. Chow
Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity / Wan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu
Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting / Narek Darabedian and Matthew R. Pratt
Optogenetic perturbation of the biochemical pathways that control cell behavior / Lauren L. Haar, David S. Lawrence and Robert M. Hughes
pH-shift stress on antibodies / Hiroshi Imamura and Shinya Honda / Small molecule target identification using photo-affinity chromatography / Seung-Yong Seo and Timothy W. Corson
Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetases / Andrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai
On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells / Ryou Kubota, Shigeki Kiyonaka and Itaru Hamachi
Bioorthogonal oncometabolite ligation / Chloe A. Briney, Susana Najera and Jordan L. Meier.