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  • Book
    edited by Akihito Yamaguchi, Kunihiko Nishino.
    Digital : Springer2018
    High Resolution Crystallographic Analysis of AcrB Using Designed Ankyrin Repeat Proteins (DARPins) -- Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter AcrB in Physiologically-Relevant Asymmetric Crystals -- Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitors -- Crystallographic Analysis of the CusBA Heavy-Metal Efflux Complex of Escherichia coli.- Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis -- NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE -- Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistance Transporter MdfA -- Biochemical Reconstitution and Characterization of Multi-Component Drug Efflux Transporters -- Covalently Linked Trimers of RND (Resistance-Nodulation-Division) Efflux Transporters to Study Their Mechanism of Action: Escherichia coli AcrB Multidrug Exporter as an Example -- Determining Ligand Path through a Major Drug Transporter, AcrB, in Escherichia coli.- Molecular Modeling of Multi-Drug Properties of Resistance Nodulation Division (RND) Transporters -- A Transcriptomic Approach to Identify Novel Drug Efflux Pumps in Bacteria -- Regulation of the Expression of Bacterial Multidrug Exporters by Two-Component Signal Transduction Systems -- Study of the Expression of Bacterial Multidrug Efflux Pumps in Anaerobic Conditions -- Identification of a Staphylococcus aureusEfflux Pump Regulator Using a DNA-Protein Affinity Technique -- High Throughput Flow Cytometry Screening of Multidrug Efflux Systems -- Single-Molecule Analysis of Membrane Transporter Activity by Means of a Microsystem -- Large Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria -- Reconstitution and Transport Analysis of Eukaryotic Transporters in the Post-Genomic Era.