Book[edited by] Mark Hochstrasser.
Contents:
Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkage / Michael Morgan, Muhammad Jbara, Ashraf Brik and Cynthia Wolberger
Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extension / Daniel C. Scott and Brenda A. Schulman
A genetic approach to study polyubiquitination in Saccharomyces cerevisiae / Fernando Meza-Gutierrez, Deniz Simsek and David P. Toczyski
Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteins / Viduth K. Chaugule, Connor Arkinson, Rachel Toth and Helen Walden
Methods to measure ubiquitin chain length and linkage / Fumiaki Ohtake, Hikaru Tsuchiya, Keiji Tanaka and Yasushi Saeki
Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBE / Yukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya and Keiji Tanaka
Methods to study phosphoribosylated ubiquitin ligation and removal / Jiazhang Qiu and Zhao-Qing Luo
Biochemical characterization of SUMO-conjugating enzymes by in vitro sumoylation assays / Nathalie Eisenhardt, Dragana Ilic, Easa Nagamalleswari and Andrea Pichler
Methods to study SUMO dynamics in yeast / Stefan Pabst, Lennard-Maximilian Döring, Natasha Petreska and R. Jürgen Dohmen
Approaches for investigating the extracellular signaling function of ISG15 / Caleb D. Swaim, Larissa A. Canadeo and Jon M. Huibregtse
Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10 / Annette Aichem, Annika N. Boehm, Nicola Catone, Gunter Schmidtke and Marcus Groettrup
Methods to analyze STUbL activity / Emma Branigan, Anna Plechanovová and Ronald T. Hay
Quantitative analysis of USP activity in vitro / Shreya Dharadhar, Robbert Q. Kim, Michael Uckelmann and Titia K. Sixma
Evaluating enzyme activities and structures of DUBs / Jonathan N. Pruneda and David Komander
Purification and functional characterization of the DUB domain of SdeA / Kedar Puvar, Shalini Iyer, Michael J. Sheedlo and Chittaranjan Das
Profiling DUBs and Ubl-specific proteases with activity-based probes / Paul P. Geurink, Gerbrand J. van der Heden van Noort, Monique P.C. Mulder, Robert C.M. Knaap, Marjolein Kikkert and Huib Ovaa
Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissues / Kathrin Kunz, Stefan Müller and Luca Mendler.