Bookedited by Dr. Naidong Weng and Dr. Wenying Jian, Janssen Research & Development, LLC.
Contents:
Part I: Overview. Overview of targeted quantitation of biomarkers and its applications / Naidong Weng
Translational application of biomarkers / Ray Bakhtiar
Current regulatory guidance pertaining biomarker assay establishment and industrial practice of fit-for-purpose and tiered approach / Naidong Weng
Modern liquid chromatography and mass spectrometry for targeted biomarker quantitation / Wenying Jian
Comparison between LC-MS and ligand-binding assay approaches for biomarker quantification / QingQing Wang, Lili Guo, and Ian A. Blair
Sample preparation methods for targeted biomarker quantification by LC-MS / Shichen Shen, Bo An, and Jun Qu
Overcome the endogenous levels in biomarker quantitation using LC-MS / Guowen Liu
Part II: Challenges and approaches. Sample collection for targeted biomarker quantitation by LC-MS / Yuzhong Deng and Xiaorong Liang
Nonspecific binding in LC-MS bioanalysis / Aimin Tan and John C. Fanaras
Strategies for improving sensitivity for targeted quantitation by LC-MS / Long Yuan and Qin C. Ji
Strategies to improve specificity for targeted biomarker quantitation by LC-MS / Yuan-Qing Xia and Jeffrey D. Miller
Biomarker quantitation using relative approaches / Shane M. Lamos and Katrina E. Wiesner
Part III: Applications. Targeted quantification of amino acid biomarkers using LC-MS / Barry R. Jones, Raymond F. Biondolillo, and John E. Buckholz
Targeted quantification of peptide biomarkers: a case study of amyloid peptides / Lieve Dillen, Marc De Meulder, and Tom Verhaeghe
Targeted protein biomarker quantitation by LC-MS / Yongle Pang, Chuan Shi, and Wenying Jian
Glycoprotein biomarkers / Shuwei Li, Stefani N. Thomas, and Shuang Yang
Targeted lipid biomarker quantitation using liquid chromatography-mass spectrometry (LC-MS) / Ashkan Salamatipour, Ian A. Blair, and Clementina Mesaros
Targeted LC-MS quantification of androgens and estrogens for biomarker development / Daniel Tamae
Steroid biomarkers / Mike (Qingtao) Huang, Shefali Patel, and Zhongping (John) Lin
Bile acids as biomarkers / Clara John, Philipp Werner, Joerg Heeren, and Markus Fischer
Biomarkers for vitamin status and deficiency: LC-MS based approach / Stanley (Weihua) Zhang and Jonathan Crowther
Quantitation of acyl-coenzyme A thioesters as metabolic biomarkers / Nathaniel Snyder
Neurotransmitter biomarkers / Guodong Zhang
Targeted quantification of carbohydrate biomarkers using LC-MS / Cong Wei and Hong Gao
Nucleoside/nucleotide biomarkers / Guodong Zhang
LC-MS of RNA biomarkers / Michael G. Bartlett, Babak Basiri, and Ning Li.