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- BookJack C. de la Torre.Contents:
Chapter 1. How does Alzheimer's begin and who gets it?
Chapter 2. Forming memories
Chapter 3. Masquerading as dementia
Chapter 4. Alzheimer's
then and now
Chapter 5. Unproven hypotheses on the cause of Alzheimer's
Chapter 6. Other hypotheses on the cause of Alzheimer's disease
Chapter 7. Alzheimer noise
Chapter 8. Social contract and Alzheimer's
Chapter 9. Genetics of Alzheimer's
Chapter 10. Powering the brain
Chapter 11. Pharmaceuticals and Alzheimer's
Chapter 12. Alzheimer vascular risk factors
Chapter 13. The Good, The Bad and The Ugly of Advanced Aging
Chapter 14. A Personal Account of How a Scientific Hypothesis Blooms Into a Life of Its Own
Chapter 15. Clinical tools to detect and predict individuals at risk of Alzheimer's
Chapter 16. The turning point for Alzheimer's
Chapter 17. How poor brain blood flow promotes Alzheimer's disease
Chapter 18. Interventions that may increase cerebral blood flow
Chapter 19. Great Expectations
Chapter 20. A road to new thinking. - ArticleMehlman IJ, Eide EL, Sanders AC, Fishbein M, Aulisio CC.J Assoc Off Anal Chem. 1977 May;60(3):546-62.Surveillance for dysentery-related invasive potential in bacteria using the Sereny keratoconjunctivitis test is restricted by expense, time factor, and necessity for confirmation. Primary screening of isolates in a standardized mammalian cell culture system is recommended. Bacteria are grown 20 hr in veal infusion, washed, and resuspended in 20% heat-inactivated fetal bovine serum (FBS) supplemented with 0.12% brain heart infusion and 0.1% bile salts. The HeLa culture is grown 20 hr as a monolayer in chamber slides with 90% minimal essential medium (MEM)-10% FBS. The host culture is infected at a ratio of 10 bacteria/mammalian cell for 3 hr at 35 degrees C. The infection medium is replaced with MEM-FBS supplemented with 300 microng lysozyme and 5 microng gentamycin/ml. The infected monolayer is incubated 5 hr at 35 degrees C to permit intracellular multiplication. Specimens are washed, fixed with methanol, and stained successively with May-Grunwald and Giemsa dyes. Bacteria occur within the cytoplasm if invasion has occurred. The criterion for a positive test is that 1% of the host cells possesses at least 5 bacteria in 2 of 3 trials. Invasiveness is correlated with and possibly preconditioned by cytotoxic principle(s). Infectivity rates vary from 0 to 30%. The cytopathic effect is noted in 5-50% of HeLa cells. Positive results must be confirmed by the Sereny test.