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- DatabaseSummary: Standards from various organizations: ANSI (ISO and IEC), ASCE, ASME, AAMI , DIN, ICC, Federal Register, Code of Federal Regulations, EU Directives.Digital Access Database
- ArticleBlackwell CM, Scarlett FA, Turner JM.J Gen Microbiol. 1977 Jan;98(1):133-9.Induction of ethanolamine ammonia-lyase formation in Escherichia coli required both the ethanolamine and vitamin B12, and was gratuitous during growth on glycerol. Ethanolamine analogues inhibited enzyme activity and inhibited growth with ethanolamine as the the nitrogen source, but did not act as inducers. Enzyme formation was more rapid when ethanolamine was added to cultures containing vitamin B12 rather than the reverse. Enzyme formation was subject to catabolic repression, glucose and acetate being particularly effective. Chloramphenicol, I-aminopropan 2-01 and 1,3-diaminopropan-2-01 prevented enzyme induction. Ethanolamine ammonia-lyase, resolved from its cobamide coenzyme, was purified 35-fold. The apoenzyme was stable for several days in the presence of ethanolamine, dithiothreitol, glycerol and K+ ions. Enzyme formation therefore requires both substrate and cobamide coenzyme to be present simultaneously as inducers.
- BookMinistério da Indústria e do Comércio-MIC, Conselho Nacional do Alcool-CNAL, Comissão Executiva Nacional do Alcool-CNAL.Print [1979]