Bookedited by Jeffrey L. Bose.
Contents:
Understanding staphylococcal nomenclature / Christina N. Krute and Jeffrey L. Bose
Restriction-modification systems as a barrier for genetic manipulation of Staphylococcus aureus / Marat R. Sadykov
Complementation plasmids, inducible gene-expression systems, and reporters for Staphylococci / Ralph Bertram
De novo assembly of plasmids using yeast recombinational cloning / Ameya A. Mashruwala and Jeffrey M. Boyd
Splicing by overlap extension PCR to obtain hybrid DNA products / Justin A. Thornton
Method for preparation and electroporation of S. aureus and S. epidermidis / Melinda R. Grosser and Anthony R. Richardson
Rapid isolation of DNA from staphylococcus / Kelsey L. Krausz and Jeffrey L. Bose
Bacteriophage transduction in Staphylococcus aureus : broth-based method / Kelsey L. Krausz and Jeffrey L. Bose
Bacteriophage transduction in Staphylococcus aureus / Michael E. Olson
Batch transduction of transposon mutant libraries for rapid phenotype screening in staphylococcus / Katherine L. Maliszewski
Conjugative transfer in Staphylococcus aureus / Cortney R. Halsey and Paul D. Fey
Allelic exchange / McKenzie K. Lehman, Jeffrey L. Bose, and Kenneth W. Bayles
Creation of staphylococcal mutant libraries using transposon Tn917 / Kelly C. Rice
Generation of a transposon mutant library in Staphylococcus aureus and staphylococcus epidermidis using bursa aurealis / Vijaya Kumar Yajjala ... [et al.]
Chemical and UV mutagenesis / Jeffrey L. Bose
Pulse field gel electrophoresis / Batu K. Sharma-Kuinkel, Thomas H. Rude, and Vance G. Fowler Jr.
RNA-sequencing of Staphylococcus aureus messenger RNA / Ronan K. Carroll, Andy Weiss, and Lindsey N. Shaw
Quantitative real-time PCR (qPCR) workflow for analyzing Staphylococcus aureus gene expression / April M. Lewis and Kelly C. Rice
Electrophoretic mobility shiftaAssays / Sarah E. Rowe and James P. O'Gara
Rapid amplification of cDNA ends for RNA transcript sequencing in staphylococcus / Eric Miller.