Bookedited by Rémy Bosselut, Melanie S. Vacchio.
Contents:
200 million thymocytes and I: A beginner's survival guide to t cell development
Development of [gamma][delta] T cells, the special-force soldiers of the immune system
Genetic tools to study T cell development
Assessment of T cell development by flow cytometry
Flow cytometry analysis of thymic epithelial cells and their subpopulations
Identifying the spatial relationships of thymic stromal and thymocyte subsets by immunofluorescence analysis
Purification of thymocyte and T cell subsets
Retroviral transduction of T cells and T cell precursors
Bone marrow and fetal liver radiation chimeras
In vitro analyses of T cell effector differentiation
Studying T cell development in thymic slices
FTOC-based analysis of negative selection
Reconstituted thymus organ culture
Induction of T cell development in vitro by delta-like (Dll)-expressing stromal cells
In vitro analysis of thymocyte signaling
Molecular analysis of mouse T cell receptor [alpha] and [beta] gene rearrangements
Intrathymic injection
Analysis of cell proliferation and homeostasis using EdU labeling
Characterization and isolation of human T cell progenitors
Approaches to study human T cell development
Humanized mice to study human T cell development
Using the zebrafish model to study T cell development.