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• Book

  ELISA : Methods and Protocols

  edited by Robert Hnasko.

  Digital Access Springer 2015
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• Book

  The Illicit Adventure : the Story of Political and Military Intelligence in the Middle East from 1898 to 1926

  H.V.F. Winstone.

  Print 1982
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• Article

  Comparative studies on blood serum alpha-L-fucosidases from several mammalian species.

  Villar E, Calvo P, Cabezas JA.

  Comp Biochem Physiol B. 1978;60(4):459-61.

  1. Peripheral blood serum alpha-L-fucosidases have been studied from various mammalian species: Sus scropha var domestica L. (pig), Capra hircus L. (goat), Bos taurus L. (bull, races Morucha and Charolais), Equus caballus L. (horse) and Equus asinus L. (donkey). 2. Fluorimetric and spectrophotometric procedures were used for determination of alpha-L-fucosidases. 3. alpha-L-Fucosidases were more active towards fluorescent substrates than towards chromogenic substrates. 4. pH optima values of the enzymes are: (A) 5.5 for sera from all above-mentioned species when fluorescent substrates were employed; (B) 4.0 for goat, 4.5 for bull, 5.0 for pig and 4.5-5.0 for horse and donkey sera when chromogenic substrates were used. 5. pH activity profiles are very similar for two races (Morucha and Charolais) of the same species (Bos taurus L.) and also for two species of the same genus (Equus caballus and Equus asinus L.). 6. These serum alpha-L-fucosidases are very labile under heat treatment, even at 37 degrees C.

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