Bookedited by Biji T. Kurien, R. Hal Scofield.
Contents:
Western blotting : origin and ascent of the species / W. Neal Burnette
Methods to concentrate proteins for protein isolation, proteomic, and peptidomic evaluation / J. P. Dean Goldring
Measuring protein concentration on nitrocellulose and after the electrophoretic transfer of protein to nitrocellulose / J. P. Dean Goldring
Detection of blotted proteins : not all blockers are created equal / Vishal Kothari and Suresh T. Mathews
Protein stains to detect antigen on membranes / Anil Dsouza and R. Hal Scofield
Fluorescent labeling of proteins and its application to SDS-PAGE and Western blotting / F. Javier Alba, Salvador Bartolomé, Antonio Bermúdez, and Joan-Ramon Daban
Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation / Neil W. Charter, Joe Horecka Chin-Yee Loh, Albert Doan, Tom Wehrman, and Keith R. Olson
Use of nonradioactive detection method for north and South-Western blot / Claudia Franke, Daniel Gräfe, Holger Bartsch, and Michael P. Bachmann
Immunoblotting using radiolabeled reagents for detection / Holger Bartsch, Claudia Franke, and Michael P. Bachmann
Immunoblotting of antigens : whole, strip, and new-line nitrocellulose membrane immunoblotting using the chemiluminescence technique / Yaser Dorri
Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods / Kenneth Hensley
Direct immunodetection of antigens within the precast polyacrylamide gel / Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend
Quantitative analysis of signal transduction with in-cell Western immunofluorescence assays / Vince Boveia and Amy Schutz-Geschwender
Ultrasensitive protein detection on dot blots and Western blots with semiconducting polymer dots / Fangmao Ye, Polina B. Smith, and Daniel T. Chiu
Co-detection of target and total protein by CyDye labeling and fluorescent ECL plex immunoblotting in a standard proteomics workflow / Caitriona Scaife, Ciara A. McManus, Pamela M. Donoghue, and Michael J. Dunn
Using biotinylated proteins to demonstrate immunodetection of antigens via Western blotting, dot blots, and immunohistochemistry / Thomas Millar, Ronald Knighton, and Jo-Anne Chuck
Calcium binding by Ro 60 multiple antigenic peptides on PVDF membrane / Biji T. Kurien and Michael P. Bachmann
Sequential use of immunoblots for characterization of autoantibody specificities / Holger Bartsch and Michael P. Bachmann
Nanogold immunodetection detection systems for the identification of autoantigens by Western blotting / Jacen S. Moore and R. Hal Scofield
Application of intermittent microwave irradiation to Western blot analysis / Yu-Ting Liu and Shinya Toyokuni
Visualization of unstained protein bands on PVDF / Jun Park, Masaharu Mabuchi, and Ajay Sharma
Multiplexed fluorescent immunodetection using low autofluorescence Immobilon®-FL membrane / Jun Park, Masaharu Mabuchi, and Ajay Sharma
Cold microwave-enabled protein detection and quantification / Niels Grützner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson, Stanislav Vitha, Jörg M. Steiner, and Andreas K. Holzenburg
TLC-blot (Far-Eastern blot) and its application to functional lipidomics / Takao Taki
Analysis of electroblotted proteins by mass spectrometry / Jose L. Luque-Garcia and Thomas A. Neubert
On-membrane renaturation of recombinant Ro60 autoantigen by calcium ions / Biji T. Kurien and Michael P. Bachmann
Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore / Lee Broderick Bockus and R. Hal Scofield
Purification of tryptic digests on polyvinylidene difluoride membrane / Biji T. Kurien and R. Hal Scofield
Detection of blotted proteins on nitrocellulose/PVDF membranes by Alta / Jayanta K. Pal, Shilpa J. Rao, and Dhanashri J. Godbole
Nonstripping "rainbow" and multiple antigen detection (MAD) Western blotting / Stan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang, and Sebastian B. Krajewski
Supported molecular matrix electrophoresis / Yu-ki Matsuno and Akihiko Kameyama
Parafilm-M®, an available cost-effective alternative for immuno-blot pouches / Syed M. S. Quadri
Succinylation-alcian blue staining of mucins on polyvinylidene difluoride membranes / Akihiko Kameyama, Weijie Dong, and Yu-ki Matsuno
Comparison of chemiluminescence vs. infrared techniques for detection of fetuin-A in saliva / Suresh T. Mathews, Emily Graff, Robert L. Judd, and Vishal Kothari
A novel methodology for stripping and reprobing of Western blots originally developed with colorimetric substrate TMB / Parmita Kar, Saurabh Kumar Agnihotri, Archana Sharma, Rekha Sachan, Madan Lal Bhatt, and Monika Sachdev
Other notable methods of membrane protein detection : a brief review / Biji T. Kurien and R. Hal Scofield
Nitrocellulose membrane : the new canvas / Jasmin R. Kurien and Bianca A. Kurien
Invisible ink marking in ECL membrane assays / Biji T. Kurien.