Bookedited by Kurt Pfannkuche.
Summary: This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo. Additional, protocols aim to facilitate the future highly relevant process of engineering cells for specific purposes, such as generation of transgenic embryos and development of hybrid cells, in order to tackle specific tasks in cell biology and medicine. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Cell Fusion: Overviews and Methods, Second Edition serves as an ideal reference on cell fusion and laboratory protocols.
Contents:
State of the art in cell-cell fusion / Lena Willkomm and Wilhelm Bloch
Fusion in cancer : an explanatory model for aneuploidy, metastasis formation, and drug resistance / Marieke Mohr, Kurt S. Zaenker, and Thomas Dittmar
Mouse embryos' fusion for the tetraploid complementation assay / Marina Gertsenstein
Generation of mouse chimeras with high contribution of tetraploid embryonic stem cells and embryonic stem cell-fibroblast hybrid cells / Natalia M. Matveeva, ElenaA. Kizilova, and Oleg L. Serov
Microfluidic systems for cell pairing and fusion / Burak Duraand Joel Voldman
Chromosome tracking in fused cells by single nucleotide polymorphisms / Azra Fatima, Jürgen Hescheler, and Tomo Šarić
Fusion of mesenchymal stem cells and islet cells for cell therapy / Shoichiro Sumi and Goichi Yanai
Detection of fusion events in mammalian skeletal muscle / Frank Suhr
FISH detection of X and Y chromosomes in combination with immunofluorescence to study contribution of transplanted cells to skeletal muscle fibers / Anna Strömberg and Monika Jansson
Using phosphatidylserine exposure on apoptotic cells to stimulate myoblast fusion / Amelia E. Hochreiter-Hufford, Sanja Arandjelovic, and Kodi S. Ravichandran
Tracing myoblast fusion in Drosophila embryos by fluorescent actin probes / Shruti Haralalka and Susan M. Abmayr
Analyzing cell fusion events within the central nervous system using bone marrow chimerism / Kevin Kemp and Kelly Hares
Cell fusion between dendritic cells and whole tumor cells / Shigeo Koidoand Jianlin Gong
Membrane nanotube formation in osteoclastogenesis / Toshio Kukita ... [et al.]
Modified adherence method (MAM) for electrofusion of anchorage-dependent cells / Marko Ušajand Maša Kandušer
FRET in the analysis of in vitro cell-cell fusion by flow cytometry / Guillermo Gómez-Icazbalceta ... [et al.]
Dual split protein (DSP) assay to monitor cell-cell membrane fusion / Shuhei Nakane and Zene Matsuda
Photoconvertible fluorescent protein-based live imaging of mitochondrial fusion / Geoffrey C. Owens and David B. Edelman.