Bookedited by Biji T. Kurien, R. Hal Scofield.
Contents:
Early days of blotting / Edwin Southern
Origins of protein blotting / Harry Towbin
Western blotting : remembrance of things past / W. Neal Burnette
Simian virus 40 and protein transfer / Jaime Renart
Western blotting : an introduction / Biji T. Kurien and R. Hal Scofield
From little helpers to automation / Biji T. Kurien and Harry Towbin
Spectrophotometric methods to determine protein concentration / J. P. Dean Goldring
Solubilization of proteins : the importance of lysis buffer choice / Mandy Peach [and three others]
Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis / Der-Yen Lee and Geen-Dong Chang
Diffusion blotting : a rapid and simple method for production of multiple blots from a single gel / Ingrid Olsen and Harald G. Wiker
Multiple immunoblots by passive diffusion of proteins from a single SDS-PAGE gel / Biji T. Kurien and R. Hal Scofield
Slice blotting / Graeme Lowe
Localizing proteins by tissue printing / Rafael F. Pont-Lezica
Dot-immunobinding assay (Dot-Iba) / Sumi Surendran, Annamma Mathai, and Vishnampet Venkataraman Radhakrishnan
Analysis of antibody clonotype by affinity immunoblotting / Biji T. Kurien and R. Hal Scofield
Glycosaminoglycan blotting and detection after electrophoresis separation / Nicola Volpi and Francesca Maccari
Well-based reverse-phase protein array of formalin- fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt
Quantitative computerized western blotting in detail / Dalit Talmi-Frank, Charles L. Jaffe, and Gad Baneth
Cationic electrophoresis and eastern blotting / Engelbert Buxbaum
Miniaturized blotting system for simultaneous detection of different autoantibodies / Ulrich Canzler [and eight others]
Proteomic expressional profiling of a paraffin-embedded tissue by multiplex tissue immunoblotting / Joon-Yong Chungand Stephen M. Hewitt
Post-staining electroblotting for efficient and reliable peptide blotting / Der-Yen Lee and Geen-Dong Chang
Multistrip western blotting : a tool for comparative quantitative analysis of multiple proteins / Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin
Western blotting using PVDF membranes and its downstream applications / Setsuko Komatsu
Blotting from phastGel to membranes by ultrasound / Joseph Kost and Aharon Azagury
Western blotting of high and low molecular weight proteins using heat / Biji T. Kurien and R. Hal Scofield
Membrane strip affinity purification of autoantibodies / Biji T. Kurien
Strip immunoblotting of multiple antigenic peptides / Biji T. Kurien
Double-blotting : a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures / Françoise Lasne
Method for resolution and western blotting of very large proteins using agarose electrophoresis / Marion L. Greaser and Chad M. Warren
Immunodetection of P-selectin using an antibody to its C-terminal tag / Padmaja Mehta-D'souza
Improvements and variants of the multiple antigen blot assay-MABA : an immunoenzymatic technique for simultaneous antigen and antibody screening / Oscar Noya [and six others]
Blotting from immobilized pH gradient gels : application to total cell lysates / Harry Towbin
Immunoprecipitation : western blot for proteins of low abundance / Edward P. Trieu and Ira N. Targoff
Native electrophoresis and western blot analysis (NEWeB) : methods and applications / Ioannis N. Manoussopoulos and Mina Tsagris
Shift-western blotting : separate analysis of protein and DNA from protein-DNA complexes / Matthias Harbers
Grid-immunoblotting / Olga Yeliosof and R. Hal Scofield
Detection and quantification of protein-protein interactions by far-western blotting / Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida
Western blot analysis of adhesive interactions under fluid shear conditions : the blot rolling assay / Robert Sackstein and Robert Fuhlbrigge
Centrifuge blotting / Jinny Paul
Blotting of coomassie blue-stained proteins from PAGE gels to transparencies / Jinny Paul
B-cell ELISPOT : for the identification of antigen-specific antibody-secreting cells / Hemangi B. Shah and Kristi A. Koelsch
T cell ELISPOT : for the identification of specific cytokine-secreting T cells / Kerry M. Leehan and Kristi A. Koelsch
Membrane microplates for one- and two-color ELISPOT and FLUOROSPOT assays / Alexander E. Kalyuzhny
SDS-PAGE to immunoblot in one hour / Biji T. Kurien [and three others]
Single-cell western blotting / Syed M. S. Quadri
Protein detection by simple western analysis / Valerie M. Harris
Western blotting using microfluidics / Pothur R. Srinivas
Two-dimensional gel-based protein standardization verified by western blot analysis / Hisao Haniu [and three others]
Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase / Biji T. Kurien, Debashish Danda, and R. Hal Scofield
Other notable protein blotting methods : a brief review / Biji T. Kurien and R. Hal Scofield.