Bookedited by Gary R. Skuse and Maureen C. Ferran.
Summary: This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics, and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes, and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.
Contents:
Generating primary cultures of murine cardiac myocytes and cardiac fibroblasts to study viral myocarditis / Barbara Sherry
Enrichment of cardiomyocytes in primary cultures of murine neonatal hearts / Sreejit Parameswaran ... [et al.]
Deep sequencing of cardiac microRNA-mRNA interactomes in clinical and experimental cardiomyopathy / Scot J. Matkovich and Gerald W. Dorn II
Next-generation sequencing technology in the genetics of cardiovascular disease / Cecilia Vecoli
Computational cardiac electrophysiology : implementing mathematical models of cardiomyocytes to Simulate Action Potentials of the Heart / Michael M. Bell and Elizabeth M. Cherry
Methods of myofibrillogenesis modeling / Nancy K. Drew and Anna Grosberg
Using the mechanical bidomain model to analyze the biomechanical behavior of cardiomyocytes / Bradley J. Roth
Fabrication of a myocardial patch with cells differentiated from human-induced pluripotent stem cells / Lei Ye, Joydeep Basu, and Jianyi Zhang
Efficient differentiation of cardiomyocytes from human pluripotent stem cells with growth factors / Rajneesh Jha, Ren-He Xu, and Chunhui Xu
Isolation, culturing, and characterization of cardiac muscle cells from nonhuman primate heart tissue / Steven M. Hoynowski and John W. Ludlow
Mouse embryonic stem cell-derived cardiac myocytes in a cell culture dish / Carley Glass ... [et al.]
Cryopreservation of neonatal cardiomyocytes / Adam C. Vandergriff, M. Taylor Hensley, and Ke Cheng
Evaluation of sarcomeric organization in human pluripotent stem cell-derived cardiomyocytes / Chrishan J.A. Ramachandra and Winston Shim
Electrotonic coupled metabolic purification of chick cardiomyocytes / Winston Shim
Gene transfer into cardiac myocytes / Sarah E. Lang and Margaret V. Westfall
Analysis of 4D myocardial wall motion during early stages of chick heart development / Madeline Midgett and Sandra Rugonyi.