Bookedited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano.
Summary: This volume provides a wide range of techniques accessible to researchers in biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics. It features step-by-step, readily reproducible protocols and notes on troubleshooting.
Contents:
Bioluminescent assays for cytochrome P450 enzymes / Douglas S. Auld, Henrike Veith, and James J. Cali
Simultaneous determination of multiple CYP inhibition constants using a cocktail-probe approach / Michael Zientek and Kuresh Youdim
High-throughput mass spectrometric cytochrome P450 inhibition screening / Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel
Synthesis, characterization, and application of ¹³c-methyl isocyanide as an NMR probe of heme protein active sites / Christopher McCullough [and others]
High-throughput fluorescence assay for cytochrome P450 mechanism-based Inactivators / Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg
Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches / Qian Cheng and F. Peter Guengerich
Genetic and mass spectrometric tools for elucidating the physiological function(s) of cytochrome P450 enzymes from Mycobacterium tuberculosis / Hugues Ouellet [and others]
Escherichia coli expression-based approach for porphyrin substitution in heme proteins / Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta
Expression in Escherichia coli of a cytochrome P450 enzyme with a cobalt protoporphyrin IX porosthetic group / Wesley E. Straub [and others]
Nanodiscs in the studies of membrane-bound cytochrome P450 enzymes / A. Luthra [and others]
Rapid LC-MS drug metabolite profiling using bioreactor particles / Linlin Zhao, Besnik Bajrami, and James F. Rusling
Fluorescence-based screening of cytochrome P450 activities in intact cells / M. Teresa Donato and M. José Gómez-Lechón
Screening for cytochrome P450 reactivity with a reporter enzyme / Kersten S. Rabe and Christof M. Niemeyer
High-throughput fluorescence assay of cytochrome P450 3A4 / Qian Cheng and F. Peter Guengerich
Targeted protein capture for analysis of electrophile-protein adducts / Rebecca E. Connor [and others]
DNA shuffling of cytochrome P450 enzymes / James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam
Measurement of P450 difference spectra using intact cells / Wayne A. Johnston and Elizabeth M.J. Gillam
DNA shuffling of cytochromes P450 for indigoid pigment production / Nedeljka N. Rosic
P450 oxidoreductase : genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein / Vishal Agrawal and Walter L. Miller
LICRED : a versatile drop-in vector for rapid generation of redox-self-sufficient cytochromes P450 / Federico Sabbadin, Gideon Grogan, and Neil C. Bruce
Update on allele nomenclature for human cytochromes P450 and the human cytochrome P450 allele (CYP-allele) nomenclature database / Sarah C. Sim and Magnus Ingelman-Sundberg
Simultaneous in vivo phenotyping of CYP enzymes / Sussan Ghassabian and Michael Murray
Detection of regulatory polymorphisms : high-throughput capillary DNase I footprinting / Matthew Hancock and Elizabeth A. Shephard
Isolation of mouse hepatocytes / Mina Edwards [and others]
Highly efficient SiRNA and gene transfer into hepatocyte-like HepaRG cells and primary human hepatocytes : new means for drug metabolism and toxicity studies / Véronique Laurent [and others].