Search

Search Results

• Book

  Target identification and validation in drug discovery : methods and protocols

  edited by Jürgen Moll, Riccardo Colombo.

  Contents:
  The path to oncology drug target validation : an industry perspective
  Identification of aptamers as specific binders and modulators of cell-surface receptor activity
  The design and structure-functional properties of DNA-based immunomodulatory sequences
  SiRNA design principles and off-target effects
  Western blot evaluation of siRNA delivery by ph-responsive peptides
  High-throughput RNAi screening for the identification of novel targets
  Integration of RNAi and small molecule screens to identify targets for drug development
  CellProfiler and KNIME : Open source tools for high content screening
  PARP inhibition as a prototype for synthetic lethal screens
  Structure-based target druggability assessment
  Validating pharmacological disruption of protein-protein interactions by acceptor photobleaching FRET imaging
  Systematic analysis of complex signal transduction pathways using protein fragment complementation assays
  Reverse phase protein microarrays and their utility in drug development
  A cell culture system that mimics chronic lymphocytic leukemia cells microenvironment for drug screening and characterization
  Two-dimensional vs. Three-dimensional in vitro tumor migration and invasion assays
  Tumor spheroid-based migration assays for evaluation of therapeutic agents
  The neurosphere assay applied to neural stem cells and cancer stem cells
  Genetically engineered animal models for in vivo target identification and validation in oncology
  Target validation in mice by constitutive and conditional RNAi
  In vivo target validation by inducible RNAi in human xenograft mouse models
  Bright-field in situ hybridization methods to discover gene amplifications and rearrangements in clinical samples
  Combined microRNA in situ hybridization and immunohistochemical detection of protein markers.

  Digital Access Springer 2013
  Get Shareable Link

  View Details
• Article

  Utilization of a differential hemoglobin elution procedure as a rapid assay for identification of embryonic and adult chicken red blood cells.

  Kline K, Sanders BG.

  Comp Biochem Physiol B. 1977;58(1):103-7.

  1. A hemoglobin elution-staining procedure has been developed for distinguishing embryonic chick red blood cells from adult chicken red blood cells. 2. Adult hemoglobin is eluted from red blood cells with 1.9 M potassium phosphate buffer, pH 7.2; whereas, embryonic hemoglobin is retained within the cells and gives positive staining with erythrosin B. 3. The hemoglobin elution-staining pattern during development can be correlated with two embryonic hemoglobins as detected by polyacrylamide gel electrophoresis. 4. The series of red blood cells staining with erythrosin B correspond to the primary erythrocyte series suggesting that hemoglobin expression during development is correlated with different cell populations.

  Digital Access Access Options
  Get Shareable Link

  PubMed