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  • Book
    edited by Jürgen Moll, Riccardo Colombo.
    Contents:
    The path to oncology drug target validation : an industry perspective
    Identification of aptamers as specific binders and modulators of cell-surface receptor activity
    The design and structure-functional properties of DNA-based immunomodulatory sequences
    SiRNA design principles and off-target effects
    Western blot evaluation of siRNA delivery by ph-responsive peptides
    High-throughput RNAi screening for the identification of novel targets
    Integration of RNAi and small molecule screens to identify targets for drug development
    CellProfiler and KNIME : Open source tools for high content screening
    PARP inhibition as a prototype for synthetic lethal screens
    Structure-based target druggability assessment
    Validating pharmacological disruption of protein-protein interactions by acceptor photobleaching FRET imaging
    Systematic analysis of complex signal transduction pathways using protein fragment complementation assays
    Reverse phase protein microarrays and their utility in drug development
    A cell culture system that mimics chronic lymphocytic leukemia cells microenvironment for drug screening and characterization
    Two-dimensional vs. Three-dimensional in vitro tumor migration and invasion assays
    Tumor spheroid-based migration assays for evaluation of therapeutic agents
    The neurosphere assay applied to neural stem cells and cancer stem cells
    Genetically engineered animal models for in vivo target identification and validation in oncology
    Target validation in mice by constitutive and conditional RNAi
    In vivo target validation by inducible RNAi in human xenograft mouse models
    Bright-field in situ hybridization methods to discover gene amplifications and rearrangements in clinical samples
    Combined microRNA in situ hybridization and immunohistochemical detection of protein markers.
    Digital Access Springer 2013