Bookedited by Sandra E. Nicholson and Nicos A. Nicola.
Contents:
Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken
Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan
In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy
Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon
Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper
Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler
The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks
Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green
Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath
Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan
Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg
Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer
Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens
Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich
Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier
Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar
Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson
Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson
In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw
Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert
Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller.