Bookedited by Lotte Bjergbæk.
Contents:
DNA repair : front and center and not going away! / Vilhelm A. Bohr
Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome / Dashnamoorthy Ravi and Alexander James Roy Bishop
Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening / Lone Vedel Schøler [and others]
Establishment of the DNA repair-defective mutants in DT40 cells / Masamichi Ishiai, Emi Uchida, and Minoru Takata
Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair / Chun Tsai [and others]
Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair / Günter Speit and Andreas Rothfuss
Detection of DNA damage by comet fluorescence in situ hybridization / Wiebke Schlörmann and Michael Glei
Quantitative DNA damage and repair measurement with the yeast comet assay / Rui Oliveira and Björn Johansson
Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR / Amy M. Furda [and others]
In vitro DNA mismatch repair in human cells / Liya Gu, Charles M. Ensor, and Guo-Min Li
Eukaryotic DNA mismatch repair in vitro / Fenghua Yuan [and others]
Immuno-slot blot assay for detection of UVR-mediated DNA damage / Mahsa Karbaschi [and others]
Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay / Thomas R. Berton and David L. Mitchell
Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR / Ahmad Besaratinia and Gerd P. Pfeifer
Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies / Milica Enoiu [and others]
Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts / Puck Knipscheer [and others]
In vitro base excision repair using mammalian cell extracts / Jason L. Parsons and Grigory L. Dianov
Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro / Dolores Córdoba-Cañero, Teresa Roldán-Arjona, and Rafael R. Ariza
In vitro monitoring of base excision repair in Saccharomyces cerevisiae / Simone Moertl and Fred Ahne
Mitochondrial base excision repair assays / Ricardo Gredilla and Tinna Stevnsner
O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks / April M. Luke and Jun Nakamura
DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis / Helen E. Bryant
Quantitative amplification of single-stranded DNA / Eva-Maria Holstein and David Lydall
Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast / James M. Dewar and David Lydall
Monitoring DNA recombination initiated by HO endonuclease / Neal Sugawara and James E. Haber
Monitoring I-SceI-induced double-strand break repair in DT40 Cells / David du Plessis and Julian E. Sale
I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks / Amanda Gunn and Jeremy M. Stark
Studying repair of a single protein-bound nick in vivo using the Flp-nick system / Ida Nielsen, Anni H. Andersen, and Lotte Bjergbæk
Measurement of diversification in the immunoglobulin light chain gene of DT40 cells / Julian E. Sale
Live cell microscopy of DNA damage response in Saccharomyces cerevisiae / Sonia Silva [and others]
Use of gene targeting to study recombination in mammalian cell DNA repair mutants / Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn
In vitro rejoining of double strand breaks in genomic DNA / George Iliakis, Emil Mladenov, and Nge Cheong
In vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides / Kamal Datta [and others]
Assays of bypass replication of genotoxic lesions in cell-free extracts / Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone
Quantitative measurement of translesion DNA synthesis in mammalian cells / Omer Ziv [and others]
Detection of PCNA modifications in Saccharomyces cerevisiae / Adelina A. Davies and Helle D. Ulrich
In vitro PCNA modification assays / Joanne L. Parker and Helle D. Ulrich
Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / George Iliakis [and others]
CHK1 kinase activity assay / Hong Yan Wang and Ya Wang
Histone H2AX phosphorylation : a marker for DNA damage / Arishya Sharma, Kamini Singh, and Alexandru Almasan.