Bookedited by Vladimir Voynov, Justin A. Caravella.
Contents:
Therapeutic proteins / Dimiter S. Dimitrov
Synthetic antibody libraries / Bryce Nelson and Sachdev S. Sidhu
Construction of "phylomer" peptide libraries as a rich source of potent inhibitors of protein/protein interactions / Nadia Milech and Paul Watt
Ribosome display and screening for protein therapeutics / Damjana Kastelic and Mingyue He
Yeast display of engineered antibody domains / Qi Zhao, Zhongyu Zhu, and Dimiter S. Dimitrov
Expression, purification, and characterization of engineered antibody CH2 and VH domains / Rui Gong, Weizao Chen, and Dimiter S. Dimitrov
Engineering of affibody molecules for therapy and diagnostics / Joachim Feldwisch and Vladimir Tolmachev
Protein design for diversity of sequences and conformations using dead-end elimination / Karl J.M. Hanf
Design and generation of DVD-Ig(TM) molecules for dual-specific targeting / Enrico DiGiammarino, Tariq Ghayur, and Junjian Liu
Engineering and expression of bibody and tribody constructs in mammalian cells and in the yeast Pichia pastoris / Steve Schoonooghe
Use of E. coli for the production of a single protein / Lili Mao and Masayori Inouye
Folding engineering strategies for efficient membrane protein production in E. coli / Brent L. Nannenga and François Baneyx
Transient expression technologies : past, present, and future / Sabine Geisse and Bernd Voedisch
Stable transfection pools for large quantity of protein production / Jianxin Ye
Mammalian stable expression of biotherapeutics / Thomas Jostock and Hans-Peter Knopf
Transgenic expression of therapeutic proteins in Arabidopsis thaliana seed / Cory L. Nykiforuk and Joseph G. Boothe
Methods for chromatographic removal of endotoxin / Adam J. Lowe, Cameron L. Bardliving, and Carl A. Batt
Effectiveness of various processing steps for viral clearance of therapeutic proteins : database analyses of commonly used steps / Dana Cipriano, Michael Burnham, and Joseph V. Hughes
High-throughput quantitative N-glycan analysis of glycoproteins / Margaret Doherty [and others]
High-throughput multimodal strong anion exchange purification and N-glycan characterization of endogenous glycoprotein expressed in glycoengineered Pichia pastoris / Sujatha Gomathinayagam [and others]
Databases and tools in glycobiology / Natalia V. Artemenko [and others]
Characterization of PEGylated biopharmaceutical products by LC/MS and LC/MS/MS / Lihua Huang and P. Clayton Gough
Identification of asp isomerization in proteins by ¹⁸O labeling and tandem mass spectrometry / Jennifer Zhang and Viswanatham Katta
Monitoring of subvisible particles in therapeutic proteins / Satish K. Singh and Maria R. Toler
Size-exclusion chromatography with multi-angle light scattering for elucidating protein aggregation mechanisms / Erinc Sahin and Christopher J. Roberts
Computational methods to predict therapeutic protein aggregation / Patrick M. Buck [and others]
Coarse-grained simulations of protein aggregation / Troy Cellmer and Nicolas L. Fawzi
Chitosan-based nanoparticles as delivery systems of therapeutic proteins / Pedro Fonte [and others]
Challenges in the development and manufacturing of antibody-drug conjugates / Laurent Ducry.