Bookedited by Miep H. Helfrich, Stuart H. Ralston.
Contents:
Primary human osteoblast cultures / Jane P. Dillon [and others]
Osteoblast isolation from murine calvaria and long bones / Astrid D. Bakker and Jenneke Klein-Nulend
Rat osteoblast cultures / Isabel R. Orriss, Sarah E.B. Taylor, and Timothy R. Arnett
Isolation of primary avian osteocytes / Cor M. Semeins, Astrid D. Bakker, and Jenneke Klein-Nulend
Isolation of mouse osteocytes using cell fractionation for gene expression analysis/ Christine Halleux [and others]
Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5 / Jennifer Rosser and Lynda F. Bonewald
Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo / Rahul S. Tare [and others]
Rodent osteoclast cultures / Isabel R. Orriss and Timothy R. Arnett
Isolation and culture of primary chicken osteoclasts / Patricia Collin-Osdoby and Philip Osdoby
Isolation and purification of rabbit osteoclasts / Fraser P. Coxon, Michael J. Rogers, and Julie C. Crockett
Generation of human osteoclasts from peripheral blood / Kim Henriksen [and others]
Osteoclast formation in mouse co-cultures / Cecile Itzstein and Robert J. Van 't Hof
RANKL-mediated osteoclast formation from murine RAW 264.7 cells / Patricia Collin-Osdoby and Philip Osdoby
Transfection of osteoclasts and osteoclast precursors / Julie C. Crockett, David J. Mellis, and Adam Taylor
Analysis of signalling pathways by western blotting and immunoprecipitation / Aymen I. Idris
Analysis of transcriptional regulation in bone cells / Huilin Jin and Stuart H. Ralston
Extraction of nucleic acids from bone / Alun Hughes, Tracy L. Stewart, and Val Mann
Analysis of gene expression in bone by quantitative RT/PCR / Alun Hughes
Histomorphometry in rodents / Reinhold G. Erben and Martin Glösmann
Studying gene expression in bone by in situ hybridization / Ina Kramer [and others]
Immunostaining of skeletal tissues / Tobias B. Kurth and Cosimo De Bari
Techniques for the study of apoptosis in bone / Sudeh Riahi and Brendon Noble
Transmission electron microscopy of bone / Vincent Everts [and others]
Scanning electron microscopy of bone / Alan Boyde
Fluorescence imaging of osteoclasts using confocal microscopy / Fraser P. Coxon
Live imaging of bone cell and organ cultures / Sarah L. Dallas and Patricia A. Veno
Analysis of bone architecture in rodents using microcomputed tomography / Robert J. van 't Hof
Bone measurements by peripheral quantitative computed tomography in rodents / Jürg A. Gasser and Johannes Willnecker
Quantitative X-ray imaging of rodent bone by Faxitron / J.H. Duncan Bassett [and others]
Bioluminescence imaging of bone metastasis in rodents / Thomas J.A. Snoeks [and others]
Fourier transform infrared imaging of bone / Eleftherios P. Paschalis
Raman microscopy of bone / Simon R. Goodyear and Richard M. Aspden
The calvarial injection assay / Robert J. van 't Hof
Ovariectomy/orchidectomy in rodents / Aymen I. Idris
Mechanical properties of bone ex vivo / Simon R. Goodyear and Richard M. Aspden
Mechanical stimulation of bone cells using fluid flow / Carmen Huesa and Astrid D. Bakker
Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation / Andrew A. Pitsillides and Simon C.F. Rawlinson
In vivo mechanical loading / Roberto Lopes de Souza and Leanne Saxon.