Bookedited by Ian A. Cree.
Contents:
Xenotransplantation of breast cancers
Feeder layers: Co-culture with nonneoplastic cells
Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay
Transfection and DNA-mediated gene transfer
Analysis of drug interactions
Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy
Proteomic evaluation of cancer cells: Identification of cell surface proteins
Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction
Expression analysis of homeobox genes in leukemia/lymphoma cell lines
Flow cytometric DNA analysis of human cancers and cell lines
Angiogenesis assays
Cell migration and invasion assays
Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR
Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay
Purification of annexin V and its use in the detection of apoptotic cells
Real-time cytotoxicity assays
Differential staining cytotoxicity assay: A review
Cell sensitivity assays: The ATP-based tumor chemosensitivity assay
Cell sensitivity assays: The MTT assay
Cellular chemosensitivity assays: An overview
Isolation of endothelial cells from human tumors
Isolation of inflammatory cells from human tumours
Establishment and culture of leukemia-lymphoma cell lines
Isolation and culture of ovarian cancer cells and cell lines
Isolation and culture of squamous cell carcinoma lines
Isolation and culture of melanoma and naevus cells and cell lines
Isolation and culture of colon cancer cells and cell lines
Generation of lung cancer cell line variants by drug selection or cloning
Quality assurance and good laboratory practice
Elimination of mycoplasmas from infected cell lines using antibiotics
Detecting mycoplasma contamination in cell cultures by polymerase chain reaction
Cell culture contamination
Cytogenetic analysis of cancer cell lines
Online verification of human cell line identity by STRDNA typing
Characterization and authentication of cancer cell lines: An overview
Storage of cell lines
Principles of cancer cell culture
Cancer biology.