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- Bookby Lynda J. Campbell.Summary: [Publisher-supplied data] Cytogenetic studies of malignancy have become an essential tool in the clinical management of cancer patients. Cancer Cytogenetics: Methods and Protocols presents eminently practical key cytogenetic and FISH techniques for every stage of diagnostic service. Experts in the field describe detailed cytogenetic analysis methods, fluorescence in situ hybridization and array methods currently being applied to investigate and diagnose different varieties of cancer. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory protocols. The authors of the various chapters have also provided extensive notes to guide individuals who are new to these methods through the pitfalls that bedevil all such testing. Authoritative and accessible, Cancer Cytogenetics: Methods and Protocols serves as an ideal guide to scientists of all backgrounds, allowing them to either establish new techniques in their laboratories or find the different variations of standard methods helpful in improving their results.
Contents:
Introduction / Lynda J. Cambell
Evolution of cytogenetic methods in the study of cancer / Lynda J. Campbell
Fluorescence in situ hybridization methods and troubleshooting applied to fixed cell suspensions / John Swansbury, Toon Min, and Shilani Aruliah
Chronic myeloid leukemia : cytogenetic methods chronic myeloid leukemia : cytogenetic methods / Christine M. Morris
Cytogenetic analysis in acute myeloid leukaemia / Lynda J. Campbell and Joanne S. White
Cytogenetics in myelodysplastic syndromes / Kazuma Ohyashiki, Atsushi Kodama, and Junko H. Ohyashiki
Cytogenetics of myeloproliferative neoplasms / Lynda J. Campbell
Acute lymphoblastic leukaemia / Claire Schwab and Christine J. Harrison
Cytogenetic methods in chronic lymphocytic leukemia / Claudia Haferlach and Ulrike Bacher
Genetic abnormalities in non-hodgkin's lymphoma as revealed by conventional and molecular cytogenetics methods of analyses / Suresh C. Jhanwar and Ryan C. Denley
Metaphase cytogenetic techniques in multiple myeloma / Jeffrey R. Sawyer
Detection of chromosome abnormalities using cytoplasmic immunoglobulin staining and FISH in myeloma / Bruce R. Mercer and Kathleen C. Rayeroux
Cytogenetics of solid tumours / Robyn Lukeis and Mary Suter
Fluorescence in situ hybridization on formalin-fixed, paraffin-embedded tissue sections / Adrian Zordan
Use of M-FISH and M-BAND to define chromosome abnormalities / Ruth N. MacKinnon and Ilse Chudoba
Utility of array comparative genomic hybridization in cytogenetic analysis / Rashmi R. Singh, K-John J. Cheung, and Douglas E. Horsman
Single nucleotide polymorphism microarray analysis of genetic alterations in cancer / Charles G. Mullighan
Cytogenetic analysis and reporting / Lynda J. Campbell.Digital Access Springer 2011 - ArticleJandial V, Horne CH, Glover RG, Nisbet AD, Campbell DM, MacGillivray I.Acta Genet Med Gemellol (Roma). 1979;28(4):319-25.In this study of 53 twin pregnancies, the plasma concentrations of the placental proteins, placental lactogen (hPL), and pregnancy-specific glycoprotein (SP1) were measured. Placental lactogen was found to be of more value than SP1 both in the detection of twins and in assessment of fetal growth. Serial measurement of either protein would seem to be more useful in assessment of fetal growth than single measurements. Preliminary studies of the less well known placental protein, PP5, in a small series of twin pregnancies indicate that it may also prove to be of clinical value in the detection and monitoring of twin pregnancies.