Bookedited by R. Paul Jarvis.
Contents:
Vol. 1. Screening or selection for chloroplast biogenesis mutants of Arabidopsis, following chemical or insertional mutagenesis / Enrique López-Juez and Alison Hills
Analysis of plastid number, size, and distribution in Arabidopsis plants by light and fluorescence microscopy / Kevin Pyke
Immunofluorescence microscopy for localization of Arabidopsis chloroplast proteins / Stanislav Vitha and Katherine W. Osteryoung
Transient expression and analysis of chloroplast proteins in Arabidopsis protoplasts / Dong Wook Lee and Inhwan Hwang
Visualisation of stromules on Arabidopsis plastids / John C. Gray, James A. Sullivan, and Christine A. Newell
Analysis of chloroplast movement and relocation in Arabidopsis / Masamitsu Wada and Sam-Geun Kong
Studying starch content and sedimentation of amyloplast statoliths in Arabidopsis roots / John Stanga, Allison Strohm, and Patrick H. Masson
Studying Arabidopsis chloroplast structural organisation using transmission electron microscopy / Stefan Hyman and R. Paul Jarvis
Transplastomics in Arabidopsis : progress toward developing an efficient method / Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga
Isolation, quantification, and analysis of chloroplast DNA / Beth A. Rowan and Arnold J. Bendich
Measurement of transcription rates in Arabidopsis chloroplasts / Yan O. Zubo, Thomas Börner, and Karsten Liere
Studying the structure and processing of chloroplast transcripts / Alice Barkan
In vitro RNA-binding assay for studying trans-factors for RNA editing in chloroplasts / Toshiharu Shikanai and Kenji Okuda
Studying translation in Arabidopsis chloroplasts / Paolo Pesaresi
Studying proteases and protein turnover in Arabidopsis chloroplasts / Lars L. Söjgren and Adrian K. Clarke
In silico methods for identifying organellar and suborganellar targeting peptides in Arabidopsis chloroplast proteins and for predicting the topology of membrane proteins / Sandra K. Tanz and Ian Small
Rapid isolation of Arabidopsis chloroplasts and their use for in vitro protein import assays / Henrik Aronsson and R. Paul Jarvis
Energetic manipulation of chloroplast protein import and the use of chemical cross-linkers to map protein-protein Interactions / Hitoshi Inoue, Fei Wang, Takehito Inaba, and Danny J. Schnell
Isolation of Arabidopsis thylakoid membranes and their use for in vitro protein insertion or transport assays / Thomas Bals and Danja Schünemann
Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction / Chiung-Chih Chu and Hsou-min Li
Studying Arabidopsis envelope protein localization and topology using thermolysin and trypsin proteases / John Froehlich. Vol. 2. One- and two-dimensional blue native-PAGE and immunodetection of low-abundance chloroplast membrane protein complexes / Shingo Kikuchi, Jocelyn Bédard, and Masato Nakai
Analysis of thylakoid protein complexes by two-dimensional electrophoretic systems / Sari Sirpiö, Marjaana Suorsa, and Eva-Mari Aro
Preparation of multiprotein complexes from Arabidopsis chloroplasts using tandem affinity purification / Charles Andrès, Birgit Agne, and Felix Kessler
Studying interactions between chloroplast proteins in intact plant cells using bimolecular fluorescence complementation and Förster resonance energy transfer / Jodi Maple and Simon G. Møller
Studying chloroplast protein interactions in vitro : an overview of the available methods / Joanna Tripp and Enrico Schleiff
Proteome databases and other online resources for chloroplast research in Arabidopsis / Diogo Ribeiro Demartini, Célia Regina Carlini, and Jay J. Thelen
Use of transcriptomics to analyze chloroplast processes in Arabidopsis / Tatjana Kleine and Dario Leister
Use of non-aqueous fractionation and metabolomics to study chloroplast function in Arabidopsis / Peter Geigenberger, Axel Tiessen, and Jörg Meurer
Chloroplast phenomics : systematic phenotypic screening of chloroplast protein mutants in Arabidopsis / Yan Lu, Linda J. Savage, and Robert L. Last
Preparation of envelope membrane fractions from Arabidopsis chloroplasts for proteomic analysis and other studies / Daniel Salvi, Lucas Moyet, Daphné Seigneurin-Berny, Myriam Ferro, Jacques Joyard, and Norbert Rolland
Preparation of stroma, thylakoid membrane, and lumen fractions from Arabidopsis thaliana chloroplasts for proteomic analysis / Michael Hall, Yogesh Mishra, and Wolfgang P. Schröder
Preparation of plastoglobules from Arabidopsis plastids for proteomic analysis and other studies / Celine Besagni, Lucia Eugeni Piller, and Claire Bréhélin
Preparation and proteomic analysis of chloroplast ribosomes / Kenichi Yamaguchi
The workflow for quantitative proteome analysis of chloroplast development and differentiation, chloroplast mutants, and protein interactions by spectral counting / Giulia Friso, Paul Dominic B. Olinares, and Klaas J. van Wijk
Use of phosphoproteomics to study posttranslational protein modifications in Arabidopsis chloroplasts / Anne Endler and Sacha Baginsky
Use of a pulse-amplitude modulated chlorophyll fluorometer to study the efficiency of photosynthesis in Arabidopsis plants / Matthew D. Brooks and Krishna K. Niyogi
Gas exchange measurements for the determination of photosynthetic efficiency in Arabidopsis leaves / Giles Johnson and Erik Murchie
Measurement of the [delta]pH and electric field developed across Arabidopsis thylakoids in the light / Steven M. Theg and Curtis Tom
Measurement of chloroplast ATP synthesis activity in Arabidopsis / Aleel K. Grennan and Donald R. Ort
Methods for analysis of photosynthetic pigments and steady-state levels of intermediates of tetrapyrrole biosynthesis / Olaf Czarnecki, Enrico Peter, and Bernhard Grimm
Analysis of starch metabolism in chloroplasts / Carmen Hostettler, Katharina Kölling, Diana Santelia, Sebastian Streb, Oliver Kötting, and Samuel C. Zeeman
Analysis of lipid content and quality in Arabidopsis plastids / Anna Maria Zbierzak, Peter Dörmann, and Georg Hölzl.