Bookedited by Gary B. Willars and R.A. John Challiss.
Contents:
Inducible expression of G protein-coupled receptors in transfected cells / Beryl Koener and Emmanuel Hermans
Using the Flp-In T-Rex system to regulate GPCR expression / Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan
Viral infection for GPCR expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi
Generation of epitope-tagged GPCRs / Yan Huang and Gary B. Willars
Use of site-directed mutagenesis to study GPCRs / Alex C. Conner [and others]
Approaches to study GPCR regulation in native systems / Jonathon M. Willets
Heterologous expression of GPCRs in fission yeast / John Davey and Graham Ladds
Radioligand binding methods for membrane preparations and intact cells / David B. Bylund and Myron L. Toews
Quantification of GPCR mRNA using real-time RT-PCR / Trond Brattelid and Finn Olav Levy
Determining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data / Christopher J. Langmead
Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells / Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill
Examining site-specific GPCR phosphorylation / Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong
Ubiquitination of GPCRs / Adriana Caballero and Adriano Marchese
[35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs / Rajendra Mistry, Mark R. Dowling, and R.A. John Challiss
Using calcium imaging as a readout of GPCR activation / Martin D. Bootman and H. Llewelyn Roderick
Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors / Frank Gesellchen [and others]
Determining the activation of Rho as an index of receptor coupling to G12/13 proteins / Michio Nakaya [and others]
Use of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events / Carl P. Nelson and R.A. John Challiss
Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs / Ashley E. Brady [and others]
Study of GPCR-protein interactions by BRET / Martina Kocan and Kevin D.G. Pfleger
Time resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization / Martin Cottet [and others]
Peptide affinity purification for the isolation and identification of GPCR-associated protein complexes / Pascal Maurice, Avais M. Daulat, and Ralf Jockers
Tandem affinity purification and identification of GPCR-associated protein complexes / Avais M. Daulat, Pascal Maurice, and Ralf Jockers
Identification of GPCR localization in detergent resistant membranes / Ranju Kumari and Anna Francesconi
Analysis of GPCR localization and trafficking / James N. Hislop and Mark von Zastrow
Statistical methods in research / Domenico Spina.